Culture Age, Temperature, and pH Affect the Polyol and Trehalose Contents of Fungal Propagules.

Applied and Environmental Microbiology
John E Hallsworth, N Magan

Abstract

The growth and conidial physiology of the entomopathogenic fungi Beauveria bassiana, Metarhizium anisopliae, and Paecilomyces farinosus were studied under different conditions. The effects of culture age (up to 120 days), temperature (5 to 35(deg)C), and pH (2.9 to 11.1) were determined. Growth was optimal at pH 5 to 8 for each isolate and between 20 and 35(deg)C, depending on the isolate. The predominant polyol in conidia was mannitol, with up to 39, 134, and 61 mg g of conidia(sup-1) for B. bassiana, M. anisopliae, and P. farinosus, respectively. Conidia of M. anisopliae contained relatively small amounts of lower-molecular-weight polyols and trehalose (less than 25 mg g(sup-1) in total) in all treatments. Conidia of B. bassiana and P. farinosus contained up to 30, 32, and 25 mg of glycerol, erythritol, and trehalose, respectively, g(sup-1), depending on the treatment. Conidia of P. farinosus contained unusually high amounts of glycerol and erythritol at pH 2.9. The apparent effect of pH on gene expression is discussed in relation to the induction of a water stress response. To our knowledge, this is the first report of polyols and trehalose in fungal propagules produced over a range of temperature or pH. Some conditions and ...Continue Reading

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Citations

Dec 9, 2003·Applied and Environmental Microbiology·John E HallsworthKenneth N Timmis
Feb 17, 2010·Journal of Industrial Microbiology & Biotechnology·Jae Su KimJong Yul Roh
Nov 26, 2009·Mycologia·Ralph A Slepecky, William T Starmer
Nov 19, 2011·FEMS Microbiology Letters·Jae Su KimBruce L Parker
Jun 24, 2015·Current Genetics·Drauzio E N RangelJohn E Hallsworth
Dec 17, 2014·The ISME Journal·Andrew StevensonJohn E Hallsworth
Aug 13, 2014·Current Genetics·Almudena Ortiz-Urquiza, Nemat O Keyhani
Dec 9, 2015·Plant Molecular Biology·Larissa BarelliMichael J Bidochka
Sep 12, 2012·World Journal of Microbiology & Biotechnology·Thet Thet MarSaisamorn Lumyong
Aug 31, 2010·Journal of Industrial Microbiology & Biotechnology·Jae Su KimBruce L Parker
Oct 21, 2009·Environmental Microbiology·Jim P Williams, John E Hallsworth
Jan 17, 2014·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Sapna Mishra, Anushree Malik
Jun 11, 2015·Environmental Microbiology Reports·Ricardo SantosJohn E Hallsworth
Nov 18, 2020·Journal of Fungi·Lan Lin, Jianping Xu
Feb 23, 2021·Journal of Basic Microbiology·Amanda R C CorvalPatrícia S Gôlo
Jul 5, 2018·World Journal of Microbiology & Biotechnology·Vivien KrellAnant V Patel
Feb 18, 2018·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Shuang YangJingsheng Liu
May 12, 2020·Fungal Biology·Claudinéia A S AraújoDrauzio E N Rangel
Feb 24, 2017·World Journal of Microbiology & Biotechnology·Facundo Muñiz-ParedesOctavio Loera

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