PMID: 7526519Jan 1, 1994Paper

Culture and characterisation of human urothelium in vivo and in vitro

Urological Research
J L PetzoldtJ R Masters

Abstract

The aim of this study was to culture human urothelium and generate enough cells for subsequent reconstructive surgery. Using a modification of the Rheinwald-Green method for the routine culture of keratinocytes from patients with burns, we successfully cultured 91% of 57 biopsies from the renal pelvis, ureter, bladder and urethra of paediatric patients. The cells could be split one to three up to 9 times at 7-10 day intervals, giving a surface area of 1000 cm2 after a 2 month culture period. Primary cultures could not be initiated in defined medium MCDB153, although cells initiated using the Rheinwald-Green method could subsequently be propagated in this medium. Cytokeratin patterns in vitro were similar to those in vivo in the expression of keratins 7, 18 and 19 (characteristic of simple epithelia) and keratin 13 (characteristic of non-cornified stratified epithelia). Cultured urothelium also expressed keratin 14 (characteristic of cornified stratified epithelium) in about 25% of cells and keratin 16 (characteristic of fast-growing cells). These findings indicate that urothelial cells can be propagated in vitro for autologous grafting, and the next step is to identify substrates suitable for urothelial cell growth and differen...Continue Reading

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Citations

Sep 7, 2000·Biochemical and Biophysical Research Communications·G BahrenbergG Jakse
Jan 1, 1995·Urological Research·J L PetzoldtJ R Masters
Jun 14, 2005·Der Urologe. Ausg. A·S MaurerA Stenzl
Mar 18, 2000·Biomaterials·J L ParienteM Le Guillou
Jun 24, 2008·Journal of Toxicology and Environmental Health. Part a·Rouslana BelikKlaus Golka
Sep 21, 2001·Environmental Health Perspectives·M R RossiD A Sens
Jan 26, 2016·Experimental Cell Research·Terry WhyardVictor Romanov
Feb 15, 2001·The Journal of Urology·T SchoellerG Wechselberger
Jan 12, 1999·British Journal of Urology·W SabbaghR A Brown
Apr 17, 2008·Environmental Health Perspectives·Seema SomjiScott H Garrett
Jul 21, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·Xu Dong ZhouDonald A Sens
Dec 12, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Zuzana DrobnáMiroslav Stýblo
Aug 1, 1996·Pediatric Surgery International·C LorenzK L Wang
Mar 20, 2020·Physiological Reviews·Marianela G DalghiGerard Apodaca
Jan 17, 2002·The Journal of Urology·Ursula K Ehmann, Martha K Terris
Jun 15, 2021·Frontiers in Cellular and Infection Microbiology·Benjamin O MurrayJennifer L Rohn

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