PMID: 26802068Jan 24, 2016Paper

Culture and Identification of Mouse Bone Marrow-Derived Dendritic Cells and Their Capability to Induce T Lymphocyte Proliferation

Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
Wenguang WangMulati Rexiati

Abstract

The aim of this study was to establish a culture method for mouse dendritic cells (DCs) in vitro and observe their morphology at different growth stages and their ability to induce the proliferation of T lymphocytes. Granulocyte-macrophage colony stimulating factor (GM-CSF) and interleukin-4 (IL-4) were used in combination to induce differentiation of mouse bone marrow (BM) mononucleocytes into DCs. The derived DCs were then assessed for morphology, phenotype, and function. The mouse BM-derived mononucleocytes had altered cell morphology 3 days after induction by GM-CSF and IL-4 and grew into colonies. Typical dendrites appeared 8 days after induction. Many mature DCs were generated, with typical dendritic morphology observed under scanning electron microscopy. Expression levels of CD11c, a specific marker of BM-derived DCs, and of co-stimulatory molecules such as CD40, CD80, CD86, and MHC-II were elevated in the mature DCs. Furthermore, the mature DCs displayed a strong potency in stimulating the proliferation of syngenic or allogenic T lymphocytes. Mouse BM-derived mononucleocytes cultured in vitro can produce a large number of DCs, as well as immature DCs, in high purity. The described in vitro culture method lays a foundati...Continue Reading

Citations

Oct 18, 2019·Frontiers in Cell and Developmental Biology·Alexander AndersohnDarren Boehning
Feb 15, 2019·Frontiers in Immunology·Marita ChakhtouraStefania Gallucci
Jun 9, 2020·BioMed Research International·Da-Hai YuanXiao-Chuan Wu
Aug 24, 2019·Frontiers in Cellular and Infection Microbiology·Yun WangHua Tang
Dec 18, 2019·Nature Biomedical Engineering·Zhaogang YangL James Lee
Jun 1, 2017·Scandinavian Journal of Immunology·N VollackS Werwitzke
Nov 15, 2020·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·R J T LebouxB Slütter
Sep 14, 2020·Allergology International : Official Journal of the Japanese Society of Allergology·Takashi KannoNaohito Ohno
Apr 10, 2021·PloS One·Elektra Kantzari RobinsonSusan Carpenter
Aug 8, 2021·International Journal of Molecular Sciences·Mohammed Yusuf ZannaMd Isa Nur Mahiza
Oct 7, 2021·American Journal of Physiology. Cell Physiology·Karatatiwant Singh SidhuDwight E Matthews

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