Culture and PCR detection of Haemophilus influenzae and Haemophilus haemolyticus in Australian Indigenous children with bronchiectasis.

Journal of Clinical Microbiology
Kim M HareHeidi C Smith-Vaughan

Abstract

A PCR for protein D (hpd#3) was used to differentiate nontypeable Haemophilus influenzae (NTHI) from Haemophilus haemolyticus. While 90% of nasopharyngeal specimens and 100% of lower-airway specimens from 84 Indigenous Australian children with bronchiectasis had phenotypic NTHI isolates confirmed as H. influenzae, only 39% of oropharyngeal specimens with phenotypic NTHI had H. influenzae. The nasopharynx is therefore the preferred site for NTHI colonization studies, and NTHI is confirmed as an important lower-airway pathogen.

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Citations

May 7, 2013·The Journal of Antimicrobial Chemotherapy·Elizabeth A WitherdenStephen G Tristram
Oct 1, 2014·Diagnostic Microbiology and Infectious Disease·Bowen ZhangStephen Tristram
Dec 19, 2014·Frontiers in Microbiology·Janessa PickeringLea-Ann S Kirkham
Mar 17, 2016·Annual Review of Pathology·Rosemary J Boyton, Daniel M Altmann
May 26, 2017·Expert Review of Respiratory Medicine·Danielle F Wurzel, Anne B Chang
Jan 31, 2014·Journal of Clinical Microbiology·Janessa PickeringHeidi Smith-Vaughan
Jun 15, 2017·Frontiers in Pediatrics·Susan J PizzuttoJohn W Upham

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