Culture conditions that influence accumulation of zwittermicin A by Bacillus cereus UW85

Applied Microbiology and Biotechnology
J L MilnerJ Handelsman

Abstract

Bacillus cereus strain UW85 produces an antibiotic, designated zwittermicin A, that is associated with the ability of UW85 to suppress damping-off disease of alfalfa (Medicago sativa) caused by the oomycete pathogen, Phytophthora medicaginis, in a laboratory bioassay. We have identified certain culture conditions that promote or suppress zwittermicin A accumulation by UW85. Maximum accumulation was detected in supernatants of trypticase soy broth cultures after sporulation, which is when cultures of UW85 provide the greatest suppression of damping-off on alfalfa. Inorganic amendments to trypticase soy broth cultures had the following effects on zwittermicin A accumulation and disease suppression: phosphate (50 mM or more) reduced zwittermicin A accumulation and disease suppression; ferric iron (0.25-1.0 mM) enhanced zwittermicin A accumulation and disease suppression; micronutrients (manganese, boron, copper, molybdenum, zinc) had no effect on zwittermicin A accumulation or disease suppression. Cultures of UW85 grown in chemically defined minimal medium supplemented with casein hydrolysate or grown in defined medium containing the minimal requirements for growth supplemented with five amino acids (Gln, Arg, Met, Phe, Ile) accum...Continue Reading

References

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Citations

Nov 6, 2009·Bioprocess and Biosystems Engineering·Khanh Dang VuRao Y Surampalli
Feb 5, 1999·Gene·A K Dunn, J Handelsman
Oct 16, 1999·Gene·E A StohlJ Handelsman
Jul 31, 2004·Annual Review of Phytopathology·Eric B Nelson
Oct 1, 2013·Biocontrol Science·Caroline F Ajilogba, Olubukola O Babalola
Jan 9, 2004·Applied and Environmental Microbiology·Elizabeth A B EmmertJo Handelsman
Sep 10, 2005·Applied and Environmental Microbiology·Stéphane CompantEssaïd Ait Barka
Aug 4, 2006·Applied and Environmental Microbiology·Snow Brook PetersonJo Handelsman
Sep 13, 2011·Bioresource Technology·Yongliang Jia, Jian-Jiang Zhong
Jan 24, 2006·Microbiological Research·Mirac YilmazYavuz Beyatli
Mar 23, 2011·Critical Reviews in Microbiology·Siele CeuppensMieke Uyttendaele
Jul 20, 2010·Critical Reviews in Microbiology·Swarnalee Dutta, Appa Rao Podile
Dec 3, 1998·Applied and Environmental Microbiology·E A B EmmertJ Handelsman
Feb 13, 1999·FEMS Microbiology Letters·E A Emmert, J Handelsman
Jun 6, 2009·Environmental Technology·Khanh Dang VuR Y Surampalli
Jul 1, 1996·Journal of Bacteriology·J L MilnerJ Handelsman
Aug 1, 1996·Applied and Environmental Microbiology·J L MilnerJ Handelsman

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