PMID: 8596702Feb 1, 1996Paper

Culture-dependent expression of Na+ conductances in airway epithelial cells

Pflügers Archiv : European journal of physiology
K KunzelmannR Gregner

Abstract

According to previous studies, amiloride-sensitive (Amil+) Na+ channels are present in apical membranes of airway epithelial cells. When isolated from intact tissue and grown in primary culture or as immortalized cell lines, these cells tend to lose these Amil+ Na+ channels. The present study examines this issue in immortalized human bronchial epithelial cells (16HBE14o- cell line). The mRNA of one subunit of the Na+ channel alphahENaC) was semi-quantified by polymerase chain reaction of reverse transcribed RNA. Transcripts were significantly increased when cells were exposed to aldosterone and dexamethasone irrespective of whether grown on permeable supports or plastic. When grown on plastic dishes 16HBE14o-cells showed cAMP-dependent Cl- currents in whole-cell (WC) patch-clamp experiments, corresponding to expression of the cystic fibrosis transmembrane conductance regulator (CFTR). Na+ currents could not be detected although cells expressed significant amounts of alphahENaC as demonstrated by Northern blot analysis. In contrast, when cells were grown on permeable supports or cultured in the presence of butyrate (5 mmol/l, plastic or permeable support) or aldosterone and dexamethasone (both 1 micromol/l, plastic or permeable ...Continue Reading

References

Feb 1, 1976·In Vitro·K N Prasad, P K Sinha
Aug 13, 1991·Biochimica Et Biophysica Acta·B A OrserH O'Brodovich
Apr 1, 1990·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·D C GruenertJ H Widdicombe
Jan 1, 1991·Advances in Experimental Medicine and Biology·J DisserE Frömter
Apr 1, 1988·Physiological Reviews·H Garty, D J Benos
Oct 1, 1989·Canadian Journal of Physiology and Pharmacology·M DuszykS F Man
Oct 1, 1987·Physiological Reviews·M J Welsh
Jul 1, 1986·The American Journal of Physiology·R E SteeleJ S Handler
Jan 1, 1985·Annual Review of Cell Biology·K Simons, S D Fuller
Jan 1, 1994·American Journal of Respiratory Cell and Molecular Biology·A L CozensD C Gruenert
Apr 1, 1994·The American Journal of Physiology·T C ChinetM J Stutts
Aug 1, 1993·The American Journal of Physiology·H O'BrodovichJ Edelson
Oct 1, 1993·The American Journal of Physiology·T C ChinetM J Stutts
May 1, 1995·The American Journal of Physiology·F J McDonaldM J Welsh
Oct 1, 1994·Pflügers Archiv : European journal of physiology·K KunzelmannR Greger
Oct 1, 1994·Pflügers Archiv : European journal of physiology·T KoslowskyK Kunzelmann
Sep 11, 1993·The American Journal of Physiology·G YueS Matalon
Jan 4, 1994·Proceedings of the National Academy of Sciences of the United States of America·N VoilleyP Barbry
Aug 1, 1993·American Journal of Respiratory Cell and Molecular Biology·G R Cott, A K Rao
Jan 1, 1993·Canadian Journal of Physiology and Pharmacology·X WangH O'Brodovich
Sep 1, 1993·American Journal of Respiratory Cell and Molecular Biology·Z P FengY Berthiaume
Aug 1, 1993·Pflügers Archiv : European journal of physiology·R GregerC Normann
Feb 1, 1993·The American Journal of Physiology·D M Kaji
Nov 1, 1995·Pflügers Archiv : European journal of physiology·K KunzelmannR Greger

❮ Previous
Next ❯

Citations

Nov 1, 1995·Pflügers Archiv : European journal of physiology·K KunzelmannR Greger
Sep 1, 2005·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·S KrummW Clauss
Jul 30, 2008·Pflügers Archiv : European journal of physiology·I A GreenwoodD L Baines
Oct 18, 2008·American Journal of Respiratory Cell and Molecular Biology·Hong-Guang NieHong-Long Ji
May 11, 2007·Respiratory Physiology & Neurobiology·Dagmar SommerMartin Fronius
Nov 14, 2006·Experimental Cell Research·Claudiu Iordache, Marek Duszyk
Oct 19, 2010·Channels·Agustin Garcia-CaballeroM Jackson Stutts
Apr 9, 2005·The Journal of Biological Chemistry·Vadim ShlyonskySarah Sariban-Sohraby
Oct 18, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Bradley J SchnackenbergRichard C Kurten
Jul 1, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Claudette JeulinFrancelyne Marano
Aug 4, 1998·The American Journal of Physiology·Z BebökE J Sorscher
Oct 13, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·L GaoC J Venglarik
Jan 11, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ray A CaldwellM Jackson Stutts
Aug 5, 1998·The American Journal of Physiology·B R Grubb, R C Boucher
Apr 17, 1999·The Journal of Biological Chemistry·R SchreiberK Kunzelmann
Dec 3, 2010·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Jens BergerMartin Fronius
Jun 19, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·A DagenaisY Berthiaume

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.