Culture-free bacterial detection and identification from blood with rapid, phenotypic, antibiotic susceptibility testing

Scientific Reports
Xuyang ShiSiu-Tung Yau

Abstract

The current culture-based approach for the diagnosis of bloodstreams infection is incommensurate with timely treatment and curbing the prevalence of multi-drug resistant organisms (MDROs) due to its long time-to-result. Bloodstream infections typically involve extremely low (e.g., <10 colony-forming unit (CFU)/mL) bacterial concentrations that require a labor-intensive process and as much as 72 hours to yield a diagnosis. Here, we demonstrate a culture-free approach to achieve rapid diagnosis of bloodstream infections. An immuno-detection platform with intrinsic signal current amplification was developed for the ultrasensitive, rapid detection, identification (ID) and antibiotic susceptibility testing (AST) of infections. With its capability of monitoring short-term (1-2 hours) bacterial growth in blood, the platform is able to provide 84-minute simultaneous detection and ID in blood samples below the 10 CFU/mL level and 204-minute AST. The susceptible-intermediate-resistant AST capacity was demonstrated.

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Citations

Mar 20, 2019·Scientific Reports·Fang OuFrédérique Vanholsbeeck
Apr 3, 2019·Annual Review of Analytical Chemistry·Dong Jin ShinTza-Huei Wang
Aug 8, 2020·Frontiers in Cellular and Infection Microbiology·Antti VasalaOlli H Laitinen
Apr 28, 2020·Critical Reviews in Analytical Chemistry·Shabi Abbas Zaidi
Oct 3, 2020·Antibiotics·Matthew D SimmonsSteven Johnson

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