Culture-independent molecular analysis of bacterial diversity in uranium-ore/-mine waste-contaminated and non-contaminated sites from uranium mines.

3 Biotech
Paltu K DhalPinaki Sar

Abstract

Soil, water and sediment samples collected from in and around Jaduguda, Bagjata and Turamdih mines were analyzed for physicochemical parameters and cultured, and yet to be cultured microbial diversity. Culturable fraction of microbial community measured as Colony Forming Unit (CFU) on R2A medium revealed microbes between 10(4) and 10(9) CFU/g sample. Community DNA was extracted from all the samples; 16S rRNA gene amplified, cloned and subject to Amplified Ribosomal DNA Restriction Analysis. Clones representing each OTU were selected and sequenced. Sequence analyses revealed that non-contaminated samples were mostly represented by Acidobacteria, Bacteroidetes, Firmicutes and Proteobacteria (β-, γ-, and/or δ-subdivisions) along with less frequent phyla Nitrospira, Deferribacteres, Chloroflexi. In contrast, samples obtained from highly contaminated samples showed distinct abundance of β-,γ- and α-Proteobacteria along with Acidobacteria,Bacteroidetes and members of Firmicutes, Chloroflexi, Candidate division, Planctomycete, Cyanobacteria and Actinobacteria as minor groups. Our data represented the baseline information on bacterial community composition within non-contaminated samples which could potentially be useful for assessing ...Continue Reading

References

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Citations

Oct 5, 2015·Applied Microbiology and Biotechnology·Xun YanMin Zhao
Nov 5, 2016·Applied Microbiology and Biotechnology·Jayaraman SuriyaMuthukalingan Krishnan
Jun 21, 2017·Environmental Microbiology·Brodie SutcliffeIan T Paulsen

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Datasets Mentioned

BETA
HM469536-HM469615
HQ909283-HQ909337

Methods Mentioned

BETA
bioprecipitation
PCR
clone library construction
environmental stress

Software Mentioned

CHIMERA
Hierarchy Browser
MEGA
_ CHECK
BLAST

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