Culture of A-431 human epidermoid carcinoma cells in serum-free medium: effect of culture conditions on the binding of [125I]-epidermal growth factor

The American Journal of Anatomy
T Barka, H van der Noen

Abstract

Serum-free culture conditions that permit the continuous growth of A-431 human epidermoid carcinoma cells were developed. In Dulbecco's modified Eagle's synthetic nutritional medium (DME) supplemented with fetuin, insulin, transferrin, biotin, and oleic acid-fatty acid-free bovine serum albumin complex A-431 cells grew at a rate comparable to that observed in the presence of calf or fetal calf serum. Of the factors tested, oleic acid had the most pronounced stimulatory effect on the growth and [3H]-thymidine incorporation of A-431 cells in serum-free medium. A-431 cells have a high number of receptors for epidermal growth factor (EGF); they bind and rapidly internalize EGF. Nevertheless, EGF did not stimulate either the growth or the [3H]-thymidine incorporation of these cells. Analyses of [125I]-EGF binding data indicated that A-431 cells grown in the presence of calf serum had about 3.2-3.9 X 10(6) specific, saturable EGF receptor sites on their surface. Linear Scatchard plots indicated a single class of noninteracting receptors with an apparent equilibrium dissociation constant of about 2.8 X 10(-9) M. The average number of receptors of A-431 cells maintained in DME supplemented with only fetuin, insulin, and transferrin for...Continue Reading

References

Jan 1, 1978·Journal of Supramolecular Structure·J B Baker, D D Cunningham
Jul 15, 1975·Experientia·J M BowerI R Willshire
Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·R N FabricantG J Todaro
Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J B BakerD D Cunningham
Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·H HaiglerS Cohen
Jan 1, 1979·Annual Review of Biochemistry·G Carpenter, S Cohen
Apr 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·S Cohen, G Carpenter
Nov 1, 1975·Hoppe-Seyler's Zeitschrift für physiologische Chemie·H Gregory, I R Willshire
Oct 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·M D Hollenberg, P Cuatrecasas
Aug 1, 1980·Archives of Biochemistry and Biophysics·P Berhanu, M D Hollenberg
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G J TodaroJ E De Larco
Mar 1, 1980·Analytical Biochemistry·D Barnes, G Sato
Nov 1, 1980·The Journal of Cell Biology·R A WolfeD B McClure
Dec 1, 1980·Cell·D Barnes, G Sato

❮ Previous
Next ❯

Citations

Sep 1, 1984·Journal of Cellular Physiology·C M Stoscheck, G Carpenter
Jan 1, 1983·Journal of Cellular Biochemistry·C M Stoscheck, G Carpenter
Jun 17, 2008·Bioorganic & Medicinal Chemistry·Alberto MartínezJulia Lorenzo

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.