Culture of bovine embryos in buffalo rat liver cell-conditioned media or with leukemia inhibitory factor

Journal of Animal Science
R N FunstonG E Seidel

Abstract

Experiments were designed to study development of bovine embryos in TCM-199 medium conditioned by preculture with buffalo rat liver (BRL) cells. Conditioned media were harvested after BRL cells were cultured until confluency (CON), or for an additional 2 d with the same cells but new medium (CON-N) or the same medium (CON-S). Glucose in TCM-199 was depleted by BRL cells to different concentrations depending on coculture procedures: CON = 3.94 mM, CON-N = 1.67 mM, and CON-S = 1.11 mM glucose. In Exp. 1, development of bovine zygotes in CON-S resulted in fewer blastocysts than development in CON (10 vs 28%, P < .05); CON-N was not different from CON (26% blastocysts). Experiment 2 examined effects of moving embryos to a fresh drop of different or identical conditioned medium after culture for 3 d. Initial culture in CON-N and final culture in CON resulted in a greater (P < .01) number of blastocysts compared with the control of CON followed by CON (32 vs 19% blastocysts). This was not entirely due to changing from low to high glucose because adding glucose to CON-N after 3 d yielded only 18% blastocysts. To test the hypothesis that beneficial effects of BRL cell-conditioned media may be due to secretion of leukemia inhibiting fac...Continue Reading

Citations

Aug 1, 2002·American Journal of Reproductive Immunology : AJRI·Marie-Pierre Piccinni
Feb 3, 2005·Molecular Reproduction and Development·Morten VejlstedPoul Maddox-Hyttel
Aug 2, 2017·Reproduction in Domestic Animals = Zuchthygiene·A Kocyigit, M Cevik
Dec 9, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Gwonhwa SongThomas E Spencer
Oct 16, 2007·Gynecologic and Obstetric Investigation·Marie-Pierre Piccinni
Jan 21, 2006·Molecular Reproduction and Development·Hyun Jeong ParkJae Yong Han
Apr 2, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·G-P LiT D Bunch
Mar 4, 2000·Animal Reproduction Science·E Gomez, C Diez
Jun 5, 2021·Biology of Reproduction·Jocelyn M CuthbertAbby D Benninghoff

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