Culture of cells from two life stages of Schistosoma mansoni.

Cytotechnology
C J Bayne, D W Barnes

Abstract

The ability to culture continuously proliferating cell lines of various organisms in vitro has provided numerous advantages in experimental approaches toward the understanding of basic biology and disease. Although in vitro approaches are common in many disciplines, this methodology has proven difficult to exploit in the study of helminthic parasites. A major cause of parasitic disease, particularly in tropical countries, is the trematode Schistosoma mansoni. We have developed in vitro techniques that allow the long term maintenance of cell cultures from two stages of the life cycle of this organism, associated with its mammalian and the molluscan hosts. We have developed quantitative assays of cell survival and proliferation in our culture systems, and obtained evidence for limited proliferation in vitro. Although the cultures we have achieved thus far are useful for many kinds of experiments in vitro, development of continuously proliferating cell lines remains our goal.

References

Mar 1, 1979·Journal of Invertebrate Pathology·H H StibbsP DeWan
Jan 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·O Duvaux-MiretA Capron
Jul 1, 1992·Molecular and Biochemical Parasitology·C B ShoemakerL D Stein
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A RajkovicF M Rottman
Feb 1, 1987·International Journal for Parasitology·G C Coles, J I Bruce
Jun 1, 1986·Journal of Helminthology·G C Coles, J Fitzgerald
Nov 1, 1980·Journal of Invertebrate Pathology·S C Pan
Jun 1, 1993·Molecular and Biochemical Parasitology·M G dos ReisC B Shoemaker
Apr 1, 1965·Experimental Parasitology·J A CLEGG
Nov 1, 1992·Parasitology Today·E Pearce, J Appleton

❮ Previous
Next ❯

Citations

Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·M G IvanchenkoC J Bayne
May 25, 2010·International Journal for Parasitology·T QuackC G Grevelding
Aug 13, 2013·PLoS Neglected Tropical Diseases·Steffen HahnelThomas Quack
Mar 23, 2021·Frontiers in Immunology·Hong YouDonald P McManus

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.