Culture of endothelial cells from human placental microvessels

Cell and Tissue Research
A KacemiG Olive

Abstract

Endothelial cells are known to participate in angiogenesis, adaptation of vascular tonus and maintenance of blood fluidity in the microcirculation. To investigate these functions in the placenta, we devised a method of isolation and culture of endothelial cells from villous microvessels. In primary culture, these intraplacental endothelial cells exhibited many features observed in microvascular endothelium from other organs: spindle-shape, rosette associations, circular arrangements and confluence. In contrast to the confluent endothelial cells derived from the umbilical vein, cells from microvessels did not form a cobblestone network. After trypsin digestion of microvessels, magnetic microbeads coated with S-Endol immunoglobulin, antithrombomodulin and Ulex europaeus-I lectins were tested for sorting endothelial cells. Only the microbeads coated with antithrombomodulin allowed a suitable magnetic cell separation after trypsinization. By contrast, the microbeads coated with each of these antibodies or with lectins attached to confluent cells from the second passage. The microbeads detached from the cells at different rates. Their examination by scanning microscopy indicates that a portion of these microbeads was phagocytosed. M...Continue Reading

Citations

Feb 1, 1997·Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie·A KacémiJ C Challier
Jan 10, 2001·In Vitro Cellular & Developmental Biology. Animal·S FengE K Asem
Sep 22, 2012·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·Jae Hwan ChoHan Soo Kim
Mar 10, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·T W LydenC L Anderson

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