PMID: 6106191Jun 1, 1980Paper

Culture of hormone-dependent functional epithelial cells from rat thyroids

Proceedings of the National Academy of Sciences of the United States of America
F S Ambesi-ImpiombatoH G Coon

Abstract

Primary cultures of rat thyroid cells were made in medium supplemented with 0.1--0.5% calf serum and containing six hormones or growth factors: insulin, thyrotropin, transferrin, hydrocortisone, somatostatin, and glycyl-L-histidyl-L-lysine acetate. The FRTL strain was purified by successive colonial isolations and was found to maintain highly differentiated features (secretion into the culture medium of physiological amounts of thyroglobulin and concentration of iodide by 100-fold). The FRTL strain has been observed for more than 3 years in continuous culture. It has maintained the same biochemical and morphological characteristics that typified the primary cultures of thyroid follicular cells immediately after their enzymatic release from the rat thyroid. Thyroid epithelial cells that were grown under more conventional cell culture conditions failed to retain these specialized characteristics. We show that maintenance in vitro of these specialized functions of rat thyroid follicular cells is dependent on low serum concentrations and supplementation with hormones in the primary cultures. Our observations indicate that this culture strategem may be aplicable to the general problem of maintenance of differentiated characteristics...Continue Reading

References

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Citations

Jun 8, 1999·The Anatomical Record·A K Christensen, C M Bourne
Feb 1, 1996·Journal of Cellular Physiology·K TörnquistB Dugué
Dec 1, 1996·Journal of Cellular Physiology·M VainioK Törnquist
Apr 3, 1998·Journal of Cellular Physiology·K TörnquistE Ekokoski
Feb 1, 1990·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J PerryD Heath
Aug 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M GalottoR Cancedda
Aug 1, 1994·Journal of Cellular Physiology·S DesruisseauO Chabaud
Oct 21, 1999·Cell Biology International·E GiontiR Porta
May 1, 1993·Cell and Tissue Research·D Toussaint-DemylleS Haumont
Mar 1, 1991·Cytotechnology·T ErnstD Barnes
Feb 1, 1991·Cytotechnology·P Castagnola, R Cancedda
Jan 1, 1993·Cytotechnology·F S Ambesi-ImpiombatoF Curcio
Jul 1, 1990·Journal of Clinical Immunology·J P CoutelierB S Prabhakar
Jul 1, 1994·Clinical & Experimental Metastasis·T HoeltingQ Y Duh
Oct 1, 1995·Digestive Diseases and Sciences·N KawamuraI Kojima
Jan 1, 1996·Pflügers Archiv : European journal of physiology·Z Pajer, A Cör
Aug 1, 1985·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·D W RattnerW Silen
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Jan 1, 1986·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·J E ErrickG N Burrow
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Nov 7, 2006·Clinical & Experimental Metastasis·Arianna NicolussiAnna Coppa
Nov 17, 2009·In Vitro Cellular & Developmental Biology. Animal·Jinhyung ParkPeter C W Kim
Jan 25, 2011·Endocrine Pathology·Annalisa PiantaGiuseppe Damante
Jul 5, 2011·Archives of Pharmacal Research·Dezhi BianJiajun Zhao
Aug 30, 1989·Biochemical and Biophysical Research Communications·M V UrsiniA M Musti

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