PMID: 9557938Apr 29, 1998Paper

Culture of human main pancreatic duct epithelial cells

In Vitro Cellular & Developmental Biology. Animal
D OdaS P Lee

Abstract

Attempts to grow human pancreatic duct epithelial cells in long-term culture have proven difficult. We have developed a system of growing these cells for several passages by adapting methods used to culture dog pancreatic duct cells. Epithelial cells were enzymatically dissociated from the main pancreatic duct and plated onto collagen-coated culture inserts suspended above a human fibroblast feeder layer. After primary culture, the cells were either passaged onto new inserts or plastic tissue culture plates in the absence of collagen. Cells grown on the latter plates were maintained in a serum-free medium. Primary pancreatic duct epithelial cells grow steadily to confluence as a monolayer in the feeder layer system. After primary culture, cells passaged onto new inserts with fresh feeder layer or plastic plates and fed with serum-free medium continued to develop into confluent monolayers for up to four passages. The cells were columnar with prominent apical microvilli, sub-apical secretory vesicles, and lateral intercellular junctions resembling the morphology of normal in vivo epithelial cells. These cells were also positive for cytokeratin 19, 7, and 8 and carbonic anhydrase II, as measured by immunohistochemistry. Metabolica...Continue Reading

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Citations

May 30, 1998·In Vitro Cellular & Developmental Biology. Animal·D OdaS P Lee
Aug 11, 2000·In Vitro Cellular & Developmental Biology. Animal·J HallB Brown
Mar 29, 2007·PloS One·Anson W LowePatrick O Brown
Feb 18, 2010·Expert Opinion on Drug Discovery·Georg FeldmannAnirban Maitra
Jun 2, 2000·Gastroenterology·C D Ulrich
Apr 15, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jiri KalabisMeenhard Herlyn
Feb 21, 2002·Pancreas·Alexis B UlrichParviz M Pour
Oct 9, 2019·Pancreas·Yoshihisa MatsushitaJames R Eshleman

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