Abstract
Our purpose was to identify human ovarian extracellular matrix (ECM) components that would support in vitro culture of human ovarian tissue and be compatible with possible future clinical applications. We characterized ovarian expression of laminins and selected three laminin tripeptides for culture experiments to be compared with Matrigel, an undefined and animal-based mixture of ECM components. Expression of the 12 laminin genes was determined on transcript and protein levels using cortical tissue samples (n = 6), commercial ovary RNA (n = 1), follicular fluid granulosa cells (n = 20), and single-cell RNA-sequencing data. Laminin 221 (LN221), LN521, LN511, and their mixture were chosen for a 7-day culture experiment along with Matrigel using tissue from 17 patients. At the end of the culture, follicles were evaluated by scoring and counting from serial tissue sections, apoptosis measured using in situ TUNEL assay, proliferation by Ki67 staining, and endocrine function by quantifying steroids in culture media using UPLC-MS/MS. Approximately half of the cells in ovarian cortex expressed at least one laminin gene. The overall most expressed laminin α-chains were LAMA2 and LAMA5, β-chains LAMB1 and LAMB2, and γ-chain LAMC1. In cu...Continue Reading
References
Jan 1, 1996·Biology of Reproduction·J J Eppig, M J O'Brien
May 1, 1997·Human Reproduction·O HovattaR M Winston
Sep 9, 2004·Reproductive Biomedicine Online·Jennifer E ScottOuti Hovatta
Jun 25, 2005·Seminars in Cancer Biology·Hynda K Kleinman, George R Martin
Jul 28, 2006·Molecular Human Reproduction·Helen F Irving-RodgersRaymond J Rodgers
Sep 1, 2006·Seminars in Reproductive Medicine·Helen F Irving-Rodgers, Raymond J Rodgers
Sep 1, 2006·Seminars in Reproductive Medicine·Courtney B BerkholtzTeresa K Woodruff
Mar 11, 2008·Human Reproduction·Evelyn E TelferK Joo Thong
Sep 2, 2008·Stem Cells·Anna DomogatskayaKarl Tryggvason
Dec 25, 2009·Cell and Tissue Research·Helen F Irving-RodgersRaymond J Rodgers
Dec 3, 2010·Biology of Reproduction·Min XuTeresa K Woodruff
Jun 21, 2011·Molecular and Cellular Endocrinology·Wenjing ZhengKui Liu
Jun 20, 2012·BMC Bioinformatics·Jian YeThomas L Madden
Oct 13, 2012·Annual Review of Cell and Developmental Biology·Anna DomogatskayaKarl Tryggvason
Jan 28, 2014·Nature Communications·Sergey RodinKarl Tryggvason
May 23, 2014·Journal of Assisted Reproduction and Genetics·Monica M LarondaTeresa K Woodruff
Dec 17, 2014·Human Reproduction·Y BaertE Goossens
Nov 28, 2015·Scientific Reports·Shuo XiaoTeresa K Woodruff
Apr 27, 2016·Human Reproduction·H YinC Yding Andersen
May 24, 2016·Reproductive Biomedicine Online·Fernanda PauliniChristiani A Amorim
Jul 4, 2016·Biomaterials·Liisa K KanninenYan-Ru Lou
Nov 4, 2016·Nature·Orie HikabeKatsuhiko Hayashi
Mar 30, 2017·Nature Communications·Shuo XiaoTeresa K Woodruff
May 17, 2017·Nature Communications·Monica M LarondaRamille N Shah
Nov 18, 2017·Toxicology and Applied Pharmacology·Jie HaoPauliina Damdimopoulou
Dec 14, 2017·Journal of Assisted Reproduction and Genetics·Maria Costanza ChitiChristiani Andrade Amorim
Feb 2, 2018·Molecular Human Reproduction·M McLaughlinE E Telfer
Feb 25, 2018·Molecular & Cellular Proteomics : MCP·Emna OuniChristiani A Amorim
Apr 11, 2018·Human Reproduction·D D ManavellaM M Dolmans
Jul 12, 2018·Nature Protocols·Samira MusahDonald E Ingber
Nov 1, 2018·Nature Communications·Monica Suryana TjinKarl Tryggvason
Jul 10, 2019·Human Reproduction·S E PorsS G Kristensen
Mar 4, 2020·Nature Communications·Magdalena WagnerPauliina Damdimopoulou
Mar 15, 2019·Molecular & Cellular Proteomics : MCP·Emna OuniChristiani A Amorim