PMID: 7088183Jul 22, 1982Paper

Culture of macrophage cell lines from normal mouse bone marrow

J CinátlM Elleder


The use of semisolid medium for the culture and cloning of haematopoietic cells has helped our understanding of their proliferation and differentiation. It has been shown that mixed colonies of granulocytes and macrophages developed, in the presence of colony-stimulating factor (CSF), from their common precursor, granulocyte-macrophage colony-forming cells (GM-CFC). Attempts at recloning these colonies in semisolid medium suggested that granulocytes and macrophages were differentiated cells incapable of further proliferation. However, our studies on cultures of larger numbers of cells demonstrate that while this may be the case for granulocytes, macrophages seem to be capable of long-term proliferation.


Feb 1, 1979·Journal of Cellular Physiology·D MetcalfT E Mandel
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Nov 1, 1975·The Journal of Experimental Medicine·T J GoudR van Furth
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Mar 1, 1991·Journal of Immunological Methods·C M WilsonW S Walker
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Oct 28, 2011·FEMS Immunology and Medical Microbiology·Rocío García-Rodas, Oscar Zaragoza
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Related Concepts

Bone Marrow Cells
Cell Division Phases
Mouse, Inbred ICRC
Complement Receptor Type 1
Receptors, Immunologic

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