PMID: 7088183Jul 22, 1982Paper

Culture of macrophage cell lines from normal mouse bone marrow

Nature
J CinátlM Elleder

Abstract

The use of semisolid medium for the culture and cloning of haematopoietic cells has helped our understanding of their proliferation and differentiation. It has been shown that mixed colonies of granulocytes and macrophages developed, in the presence of colony-stimulating factor (CSF), from their common precursor, granulocyte-macrophage colony-forming cells (GM-CFC). Attempts at recloning these colonies in semisolid medium suggested that granulocytes and macrophages were differentiated cells incapable of further proliferation. However, our studies on cultures of larger numbers of cells demonstrate that while this may be the case for granulocytes, macrophages seem to be capable of long-term proliferation.

References

Feb 1, 1979·Journal of Cellular Physiology·D MetcalfT E Mandel
Jan 1, 1979·Methods in Enzymology·D O Adams
Nov 1, 1975·The Journal of Experimental Medicine·T J GoudR van Furth
Jun 1, 1966·The Australian Journal of Experimental Biology and Medical Science·T R Bradley, D Metcalf
Dec 1, 1965·Journal of Cellular Physiology·D H Pluznik, L Sachs
Oct 1, 1966·Experimental Cell Research·D H Pluznik, L Sachs
Oct 1, 1980·The Journal of Experimental Medicine·T M DexterD Metcalf

Citations

Mar 1, 1991·Journal of Immunological Methods·C M WilsonW S Walker
Sep 14, 1994·Journal of Immunological Methods·W S Walker
Apr 1, 1983·Blut·G E Hübner
Oct 28, 2011·FEMS Immunology and Medical Microbiology·Rocío García-Rodas, Oscar Zaragoza
Dec 1, 1991·FEMS Microbiology Immunology·H M OgmundsdóttirB Asgeirsson
Feb 1, 1984·Journal of Cellular Physiology·D ZiporiZ Malik

Related Concepts

Bone Marrow Cells
Cell Division Phases
Granulocyte
Macrophage
Mouse, Inbred ICRC
Pinocytosis
Complement Receptor Type 1
Receptors, Immunologic

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