Culture of serum-induced spheroplasts from Vibrio cholerae.

Journal of Bacteriology
A Iannetta, R J Wedgwood

Abstract

Spheroplasts of Vibrio cholerae types Inaba, Hikojima, and Ogawa were produced in serum. An excess of lysozyme was added to expedite transformation, and CaCl(2) was used as the stabilizing agent. At the optimal time for each strain, when less than 1% classical rod forms were observed microscopically, samples were plated on both conventional and modified L-form media. No growth occurred on conventional media; L-form type colonies appeared on L-form media after 2 to 6 days of incubation. L-form colonies could be subcultured on conventional media and required from four to six passages for complete reversion to classical parent forms. Reaction mixtures of Hikojima and Inaba types were passed through membrane filters (0.45 mu); L-form colonies were grown from both strains after spheroplast transformation. Appropriate controls were negative. It is suggested that the replicative particle may be filtrable.

References

Dec 1, 1965·The Journal of Clinical Investigation·L T GutmanR J Wedgwood
Jul 1, 1963·Proceedings of the Society for Experimental Biology and Medicine·B A FREEMANW BURROWS
Jun 1, 1950·Journal of Bacteriology·L DIENESS MADOFF

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Citations

Nov 20, 1969·The New England Journal of Medicine·D S Feingold
Dec 1, 1968·Journal of Bacteriology·D S FeingoldH M Kuritz

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