Cultured endothelial cells increase their capacity to synthesize prostacyclin following the formation of a contact inhibited cell monolayer

Journal of Cellular Physiology
A EldorZ Fuks

Abstract

The synthesis of the prostaglandins (PG), prostacyclin (PGI2), PGE2, and thromboxane A2 (TXA2), has been investigated in actively growing and contact-inhibited bovine aortic endothelial cell cultures. Cells were stimulated to synthesize prostaglandins by exposure to exogenous arachidonic acid or to the endoperoxide PGH2 and by the liberation of endogenous arachidonic acid from cellular lipids with melittin or ionophore A23187. Increased capacity of the cells to synthesize PGI2 and PGE2 was observed as a function of time in culture, regardless of the type of stimulation. TXA2 production increased with time only upon stimulation of the cells with ionophore A23187. This increased PG synthetic capacity was independent of cell density since it was mainly observed in confluent, nondividing endothelial cell cultures. The fact that increased PGI2 production in confluent cells was also observed with PGH2, a direct stimulator of PGI2 synthetase, implies that this process is independent of the arachidonate concentration within the cells or in the culture medium. This increased capacity is likely to reflect an increased activity of the PG synthetase system associated with the formation of a contact inhibited endothelial cell monolayer. A s...Continue Reading

References

Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·I VlodavskyD Gospodarowicz
Nov 1, 1978·The Journal of Clinical Investigation·B B WekslerE A Jaffe
Nov 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·D GospodarowiczC Birdwell
Sep 1, 1973·Prostaglandins·S BaumingerH R Lindner
Feb 23, 1981·Biochimica Et Biophysica Acta·N K Hopkins, R R Gorman
Sep 1, 1981·Journal of Cellular Physiology·P J Del Vecchio, J R Smith
Mar 1, 1981·The Journal of Clinical Investigation·R I LevinK Tack-Goldman
Mar 1, 1980·Prostaglandins and Medicine·P MaurerE Melamed
Jun 12, 1981·Science·K V HonnA Skoff
Jan 1, 1981·Advances in Cancer Research·L Levine
Nov 1, 1981·Prostaglandins·L Taylor, P Polgar

❮ Previous
Next ❯

Citations

Sep 1, 1993·Journal of Cellular Biochemistry·A B DodgeP A D'Amore
Feb 1, 1989·Inflammation·D A DuboseH B Hechtman
Jan 1, 1990·Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie·H RixenJ Friedrich
Dec 1, 1988·Mechanisms of Ageing and Development·N HasegawaK Yamamoto
Sep 9, 1999·European Journal of Pharmacology·A SamokovliskyA Danon
Oct 23, 1997·Current Opinion in Cell Biology·M G Lampugnani, E Dejana
Jan 6, 2000·American Journal of Reproductive Immunology : AJRI·Y WangE Brown
Jan 1, 1984·The Journal of Clinical Investigation·A EldorA Panet
Jan 1, 1990·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·C J KirkpatrickC Mittermayer
Jul 1, 1991·International Journal of Radiation Biology·D B RubinW F Ward
Jun 1, 1989·Prostaglandins, Leukotrienes, and Essential Fatty Acids·A EldorD B Rubin
Jan 1, 1987·Annals of the New York Academy of Sciences·B B Weksler
Jun 1, 1987·Hypertension·A L LoebM J Peach
Oct 1, 1990·Hypertension·J J Marshall, H A Kontos
Sep 1, 1985·Hypertension·A L LoebM J Peach
Jul 1, 1997·Arteriosclerosis, Thrombosis, and Vascular Biology·C Garlanda, E Dejana
Sep 12, 1984·Biochimica Et Biophysica Acta·M D Rosenthal, J R Hill

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.