Cultured epithelial grafting using human amniotic membrane: the potential for using human amniotic epithelial cells as a cultured oral epithelium sheet

Archives of Oral Biology
Takeshi KoikeHiroshi Kurita

Abstract

Human amniotic cells are a valuable source of functional cells that can be used in various fields, including regenerative medicine and tissue engineering. The aim of this study was to investigate the utility of human amniotic epithelial (hAE) cells as a new cell source for culturing stratified epithelium sheets for intraoral grafting. Enzymatically isolated hAE cells were submerged in a serum-free, low-calcium-supplemented MCDB 153 medium without a feeder layer. The hAE cells were seeded onto a Millicell cell culture plate insert and cultured while submerged in a high-calcium medium for 4 days. Then, they were cultured at an air-liquid interface for 3 weeks. Cultures of hAE cells proliferated at the air-liquid interface. After 3 weeks, the hAE cells cultivated using the air-liquid interface method lead to almost 10 continuous layers of stratified epithelium without parakeratinization or keratinization. It confirmed immunohistochemically that the presence of CK10/13 and Ki-67 positive cells were spread throughout almost all the epithelial layer, and that CK19 positive cells were expressed throughout the entire epithelial layer in the cultured hAE cell sheets. Cultured hAE cells sheets showed a staining pattern similar to that of...Continue Reading

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Citations

Jan 7, 2014·International Journal of Biological Sciences·Qing-Song ZhaoZhi-Feng Cheng
Apr 24, 2012·Journal of Biomedicine & Biotechnology·John P SoleasThomas K Waddell
Aug 19, 2014·International Journal of Oral and Maxillofacial Surgery·I B KarS Misra
Nov 26, 2015·Journal of Dermatological Science·Lei-Wei JiangHongguang Lu
Jun 26, 2014·Biotechnology Journal·Toshiyuki OwakiTeruo Okano
May 28, 2013·Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology·Tetsu ShimaneHiroshi Kurita
Jun 5, 2018·Cell Transplantation·Soraya Rasi GhaemiNicolas H Voelcker
Oct 2, 2019·International Journal of Clinical Pediatric Dentistry·N B NagaveniAshu J Soni

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