Cultured gallbladder epithelial cells synthesize apolipoproteins A-I and E

American Journal of Physiology. Gastrointestinal and Liver Physiology
Jin LeeRahul Kuver

Abstract

Gallbladder epithelial cells (GBEC) are exposed to high and fluctuating concentrations of biliary cholesterol on their apical (AP) surface. GBEC absorb and efflux cholesterol, but the mechanisms of cholesterol uptake, intracellular trafficking, and efflux in these cells are not known. We previously reported that ATP binding cassette (ABC)A1 mediates basolateral (BL) cholesterol efflux in cultured polarized GBEC. In addition, the nuclear hormone receptors liver X receptor (LXR)alpha and retinoid X receptor (RXR) mediate both AP and BL cholesterol efflux. An interesting finding from our previous study was that apolipoprotein (apo)A-I applied to the AP surfaces of cells elicited BL ABCA1-mediated cholesterol efflux. Because ABCA1-mediated cholesterol efflux requires the presence of a cholesterol acceptor, we hypothesized that GBEC synthesize and secrete endogenous apo into the BL compartment. Here, we demonstrate that cholesterol loading of cells with model bile and AP apoA-I treatment is associated with an increase in the synthesis of apoE mRNA and protein. Furthermore, apoE is secreted into the BL compartment. LXRalpha/RXR ligands stimulate the synthesis of endogenous apoA-I mRNA and protein, as well as apoE mRNA. BL secretion o...Continue Reading

References

Apr 1, 1990·Pathology·N R PattinsonB A Chapman
Feb 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·M E BoumaR Infante
May 1, 1987·Journal of Clinical Pathology·M R JacynaI A Bouchier
Nov 1, 1971·The American Journal of Medicine·H O Wheeler
Sep 30, 1994·Biochemical and Biophysical Research Communications·R KuverW R Osborne
Jul 1, 1995·The Journal of Clinical Investigation·G A FrancisJ F Oram
Apr 1, 1993·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T OhtaT Terada
Feb 1, 1997·In Vitro Cellular & Developmental Biology. Animal·R KuverS P Lee
Sep 17, 1999·The Journal of Biological Chemistry·D ReesL Kritharides
Jun 20, 2000·The Journal of Biological Chemistry·P CostetA R Tall
Aug 5, 2000·Biochemical and Biophysical Research Communications·K SchwartzD P Wade
Oct 14, 2000·Annual Review of Cell and Developmental Biology·J J Repa, D J Mangelsdorf
Dec 9, 2000·Biochimica Et Biophysica Acta·J F Oram
Jan 11, 2001·Trends in Cardiovascular Medicine·S FazioL L Swift
Jun 28, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A Von EckardsteinG Assmann
Jul 5, 2001·Gastroenterology·W G Haigh, S P Lee
Dec 1, 2001·Science·A ChawlaD J Mangelsdorf
Dec 18, 2001·Arteriosclerosis, Thrombosis, and Vascular Biology·Z H HuangT Mazzone
Mar 20, 2002·The Journal of Biological Chemistry·Joyce J RepaDavid J Mangelsdorf
Jul 9, 2002·Journal of Gastroenterology and Hepatology·Magnus S C JohnsonBjörn Carlsson
Oct 3, 2002·Biochemical and Biophysical Research Communications·Edward B NeufeldH Bryan Brewer

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Citations

Sep 6, 2011·Biochimica Et Biophysica Acta·Sophie StukasCheryl L Wellington
Jan 12, 2010·Clinica Chimica Acta; International Journal of Clinical Chemistry·Yan-Wei HuQian Wang
Jul 3, 2010·Journal of Gastroenterology and Hepatology·Beom Jae LeeSeikwan Oh
Jul 25, 2003·Biochemical and Biophysical Research Communications·Aimee Tauscher, Rahul Kuver
Mar 8, 2005·Matrix Biology : Journal of the International Society for Matrix Biology·C GentiliF Descalzi Cancedda
Aug 27, 2016·Gut and Liver·Kyo-Sang YooHo Soon Choi
Sep 18, 2004·Journal of Lipid Research·Benjamín ErranzMaría-Paz Marzolo
Dec 17, 2014·Laboratory Investigation; a Journal of Technical Methods and Pathology·Yongsheng ChenShuodong Wu

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