PMID: 6170653Nov 1, 1981Paper

Cultured human amniotic fluid cells characterized with antibodies against intermediate filaments in indirect immunofluorescence microscopy

The Journal of Clinical Investigation
I VirtanenP Aula

Abstract

Cells cultured from second trimester human amniotic fluid were characterized in indirect immunofluorescence (IIF) microscopy using specific antibodies against the subunit proteins of different types of cytoskeletal intermediate filaments. Most of the amniotic fluid cell cultures contained only epithelial cells as indicated by the positive keratin-fluorescence in IIF. Five distinct types of keratin-positive cells could be characterized. A dominating cell type (E-1) in most cultures were rapidly proliferating epithelial cells, previously called amniotic fluid cells (AF-cells). These cells showed a fibrillar cytoplasmic fluorescence both with keratin antibodies and with antibodies against vimentin, the fibroblast type of intermediate filament protein. E-1 cells did not show the typical cell-to-cell arrangement of keratin fibrils between the adjacent cells, a characteristic previously found in most cultured epithelial cells. Most of the cultures also contained large epitheloid cells (E-2), showing a fine fibrillar cytoplasmic organization of both keratin- and vimentin filaments, clearly different from that seen in E-1 cells. Several cultures contained two additional epithelial cells both showing the typical cell-to-cell arrangement...Continue Reading

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Citations

Apr 30, 1985·Clinica Chimica Acta; International Journal of Clinical Chemistry·L RisteliJ Risteli
Nov 1, 1983·Developmental Biology·E LehtonenI Virtanen
Apr 1, 1984·Journal of Steroid Biochemistry·D J O'ShannessyJ H Priest
Dec 1, 1983·Journal of the Neurological Sciences·I ElovaaraJ Palo
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·F C RamaekersG P Vooijs
Sep 1, 1984·The Journal of Cell Biology·I VirtanenV P Lehto
Aug 25, 1988·Nucleic Acids Research·C M SaxZ E Zehner
Oct 1, 1983·Pediatric Dermatology·K A Holbrook
Feb 24, 2004·The British Journal of Ophthalmology·A JosephH S Dua
Jan 1, 1983·Journal of Clinical Pathology·D J EvansM Jacobs
Sep 4, 2003·Journal of Perinatal Medicine·Nicole Ochsenbein-KölbleRoland Zimmermann
Jan 1, 1984·Human Genetics·H von KoskullI Virtanen
Jan 1, 1985·Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie·S StenmanK von Smitten
Jan 1, 1988·Histochemistry·M KasperU Karsten
Jan 1, 1983·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·M MiettinenI Virtanen
Aug 1, 1982·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·M MiettinenI Virtanen
Jan 1, 1987·Cancer Metastasis Reviews·A Raz, A Ben-Ze'ev
Feb 1, 1986·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·K VettenrantaK O Raivio
Nov 17, 2010·Izvestiia Akademii nauk. Seriia biologicheskaia·D A Davydova
Jan 1, 1987·Virchows Archiv. A, Pathological Anatomy and Histopathology·S C Henzen-LogmansC J Meijer
Jan 1, 1986·Virchows Archiv. A, Pathological Anatomy and Histopathology·H HoeflerP U Heitz
Jun 1, 2011·Expert Opinion on Biological Therapy·Petra A B KlemmtBernd Groner
Jun 16, 2016·Seminars in Pediatric Surgery·Elizabeth MaughanPaolo De Coppi
Apr 1, 1985·Kidney International·E von WillebrandP Häyry
Nov 1, 1983·The British Journal of Dermatology·A L KariniemiI Virtanen
Jan 1, 1983·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·V P LehtoI Virtanen
Mar 7, 2017·Reproductive Biology·Adila A HamidNorshariza Nordin

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