PMID: 8962090Dec 10, 1996Paper

Cultured melanocytes from dilute mutant mice exhibit dendritic morphology and altered melanosome distribution

Proceedings of the National Academy of Sciences of the United States of America
D William ProvanceJohn A Mercer

Abstract

Mutant alleles at the dilute unconventional myosin heavy chain locus cause diluted coat color, opisthotonic seizures, and death. The dilute coat color phenotype is caused by irregular clumping of pigment in the hair, but amounts of melanin are unchanged from wild-type controls. The melanocyte phenotype has been described as adendritic, since hair bulb and Harderian gland melanocytes appear to be rounded in tissue sections. These observations do not exclude the possibility that the processes lack pigment, since the melanocyte shape was judged by the distribution of melanin. We have tested this hypothesis by culturing primary melanocytes from dilute mutant and wild-type mice. The mutant melanocytes do not lack processes; instead, they exhibit a concentrated perinuclear distribution of melanosomes, while wild-type melanocytes have a very uniform cytoplasmic distribution of melanosomes. Electron micrographs show no detectable differences in melanosome morphology or maturation between dilute and wild-type melanocytes. Immunofluorescence experiments indicate that the dilute protein is concentrated in regions of the cytoplasm that contain melanosomes. These experiments show that the dilute myosin is necessary for the localization of m...Continue Reading

References

May 1, 1968·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A B NovikoffL Biempica
Apr 1, 1973·Journal of Ultrastructure Research·V J HearingM A Lutzner
Nov 1, 1967·The Journal of Heredity·M A LutznerH W Jordan
Jul 1, 1983·The Journal of Heredity·H O Sweet
Nov 22, 1994·Proceedings of the National Academy of Sciences of the United States of America·W M BementM S Mooseker
Jul 5, 1994·Proceedings of the National Academy of Sciences of the United States of America·W M BementM S Mooseker
Jan 1, 1993·The Journal of Investigative Dermatology·S J OrlowS Pifko-Hirst
Jan 1, 1995·Annual Review of Cell and Developmental Biology·M S Mooseker, R E Cheney
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·L P ZhaoJ A Mercer

❮ Previous
Next ❯

Citations

Oct 19, 1999·Microscopy Research and Technique·A S DePina, G M Langford
Jul 25, 1998·Cell Motility and the Cytoskeleton·X WuJ A Hammer
Mar 29, 2002·Cell Motility and the Cytoskeleton·João Carlos da Silva BizarioEnilza Maria Espreafico
Sep 5, 2002·Cell Motility and the Cytoskeleton·Josephine Sui-Yan Au, Jian-Dong Huang
Jun 2, 2000·Biochemical and Biophysical Research Communications·T SakamotoT Ando
Jun 24, 2008·Cell and Tissue Research·Foued S EspindolaEnilza M Espreafico
Jan 17, 2002·Current Opinion in Cell Biology·John A Hammer, Xufeng S Wu
Mar 4, 1998·Current Opinion in Cell Biology·J P Baker, M A Titus
Feb 7, 1998·Current Opinion in Neurobiology·T Hasson, M S Mooseker
Aug 30, 2003·Trends in Cell Biology·Kammy L FehrenbacherLiza A Pon
Mar 1, 1997·Trends in Cell Biology·M A Titus
Apr 12, 2006·Nature Reviews. Molecular Cell Biology·Lois S Weisman
Jan 25, 2005·Nature Structural & Molecular Biology·HoaAnh Nguyen, Hideo Higuchi
Aug 22, 2000·The British Journal of Dermatology·G VancoillieJ M Naeyaert
Jun 27, 2013·Proceedings of the National Academy of Sciences of the United States of America·Zhiyi WeiMingjie Zhang
May 15, 2002·Molecular Biology of the Cell·Xufeng WuJohn A Hammer
Apr 10, 1999·Molecular Biology of the Cell·S L Reck-PetersonM S Mooseker
Jun 16, 2001·Molecular Biology of the Cell·L A LapierreJ R Goldenring
Dec 31, 1997·Molecular Biology of the Cell·A A NascimentoE M Espreafico
Oct 21, 2003·Molecular Biology of the Cell·Tracy T CaoMark S Mooseker
Mar 25, 2005·Molecular Biology of the Cell·Aniko VaradiGuy A Rutter
Nov 30, 1999·Pigment Cell Research·M C Tuma, V I Gelfand
Nov 20, 2002·Annals of the New York Academy of Sciences·Sergio D RoséJosé-María Trifaró
Mar 8, 2011·Pigment Cell & Melanoma Research·Tomohisa Hirobe
Dec 28, 1999·Annual Review of Cell and Developmental Biology·S S Brown
Oct 23, 2003·Annual Review of Cell and Developmental Biology·Alexandra A NascimentoVladimir I Gelfand
Mar 6, 2010·American Journal of Respiratory Cell and Molecular Biology·Ko-Wei LinKenneth B Adler
Dec 3, 2013·Applied Biochemistry and Biotechnology·Jong Young JoungJae Sung Hwang
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·N L Catlett, L S Weisman
Aug 16, 2001·Proceedings of the National Academy of Sciences of the United States of America·L E MatesicN A Jenkins
Jun 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·S M WilsonN A Jenkins
Jul 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·E M EspreaficoB Kachar
Jun 7, 2011·Annual Review of Cell and Developmental Biology·M Amanda HartmanJames A Spudich
Feb 3, 2005·The Journal of Cell Biology·Natasha PashkovaLois S Weisman
Aug 12, 1998·The Journal of Cell Biology·R C Stevens, T N Davis

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.