Cultured muscle cells from insulin-resistant type 2 diabetes patients have impaired insulin, but normal 5-amino-4-imidazolecarboxamide riboside-stimulated, glucose uptake

The Journal of Clinical Endocrinology and Metabolism
E A McIntyreM Walker

Abstract

Impaired insulin action is a characteristic feature of type 2 diabetes. The study aims were to investigate whether after prolonged culture skeletal muscle cultures from insulin-resistant, type 2 diabetic patients (taking >100 U insulin/d) displayed impaired insulin signaling effects compared with cultures from nondiabetic controls and to determine whether retained abnormalities were limited to insulin action by studying an alternative pathway of stimulated glucose uptake. Studies were performed on myotubes differentiated for 7 d between passages 4 and 6. Insulin-stimulated glucose uptake (100 nm; P < 0.05) and insulin-stimulated glycogen synthesis (1 nm; P < 0.01) were significantly impaired in the diabetic vs. control cultures. Protein kinase B (PKB) expression and phosphorylated PKB levels in response to insulin stimulation (20 nm) were comparable in the diabetic and control cultures. 5-Amino-4-imidazolecarboxamide riboside (AICAR) mimics the effect of exercise on glucose uptake by activating AMP-activated protein kinase. There was no difference in AICAR (2 mm)-stimulated glucose uptake between diabetic vs. control myotube cultures (P = not significant). In conclusion, diabetic muscle cultures retain signaling defects after p...Continue Reading

References

Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·H M Blau, C Webster
Oct 6, 1998·Annual Review of Biochemistry·D G HardieM Carlson
Jan 5, 1999·The Journal of Biological Chemistry·R HalseS J Yeaman
Sep 11, 2001·The Journal of Clinical Endocrinology and Metabolism·S E NikoulinaR R Henry
Mar 2, 2002·Journal of Diabetes and Its Complications·T P CiaraldiR R Henry
Jun 19, 2002·Journal of Applied Physiology·Kei Sakamoto, Laurie J Goodyear
Jun 19, 2002·Journal of Applied Physiology·Jørgen F P WojtaszewskiErik A Richter
Jul 23, 2002·Journal of Applied Physiology·Juleen R Zierath

❮ Previous
Next ❯

Citations

Jun 12, 2013·Cell and Tissue Research·Vigdis AasArild C Rustan
May 19, 2007·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Henrik GalboLuc J C van Loon
Dec 15, 2010·Journal of Clinical Pharmacology·Marlies BosselaarCees J Tack
Dec 2, 2008·Biochimica Et Biophysica Acta·Sara FröjdöLuciano Pirola
Oct 4, 2017·Journal of Physiology and Biochemistry·Hakam Alkhateeb, Esam Qnais
Dec 15, 2018·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Michael Gaster
Dec 23, 2004·American Journal of Physiology. Endocrinology and Metabolism·Anne CorbouldAndrea Dunaif
Jul 9, 2009·Physiological Reviews·Gregory R Steinberg, Bruce E Kemp
Aug 22, 2014·American Journal of Physiology. Cell Physiology·Lubna Al-KhaliliJuleen R Zierath
Jul 11, 2006·The Journal of Clinical Endocrinology and Metabolism·Gregory R SteinbergBruce E Kemp
Nov 6, 2014·American Journal of Physiology. Endocrinology and Metabolism·Audrey E BrownMark Walker
Oct 7, 2018·Pflügers Archiv : European journal of physiology·Steven Carter, Thomas P J Solomon
Oct 25, 2007·American Journal of Physiology. Endocrinology and Metabolism·Audrey E BrownMark Walker
Sep 4, 2020·Tissue Engineering. Part a·Francisca M AcostaChristopher R Rathbone
Mar 18, 2021·Diabetologia·Thiago M BatistaC Ronald Kahn

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.