Abstract
To compare histamine receptor expression in cultured smooth muscle cells from the human detrusor and internal sphincter using receptor-specific agonists. Smooth muscle cells from the bladder dome and internal sphincter were cultured from 5 male patients undergoing cystectomy for bladder cancer therapy. Calcium transients in cells stimulated with carbachol, histamine, histamine receptor 1 (H1R)-specific heptanecarboxamide (HTMT), dimaprit (H2R), and R-(alpha)-methylhistamine (H3R) were measured by calcium imaging. Histamine receptor proteins were detected by Western blot analysis and immunocytochemistry. H1R, H2R, and H3R expression was found in tissue and cultured cells. Carbachol stimulated equal numbers of detrusor and sphincter cells (60% and 51%, respectively). Histamine stimulated significantly more cells than carbachol in detrusor (100%) and sphincter (99.34%) cells. Calcium responses to carbachol in detrusor and sphincter cells were comparable and did not differ from those to histamine in detrusor cells. However, histamine and specific agonists stimulated more sphincter cells than did carbachol (P <0.001), and the calcium increase was greater in sphincter cells than in detrusor cells. Single cell analysis revealed compar...Continue Reading
References
Dec 1, 1992·American Journal of Respiratory Cell and Molecular Biology·C FlorioS Heisler
Nov 1, 1990·British Journal of Pharmacology·R W HewS J Hill
Jul 1, 1991·The Journal of Urology·S BeneditoA García-Sacristán
Jul 1, 1987·Japanese Journal of Pharmacology·K HondaC Nakagawa
Apr 1, 1988·Agents and Actions·E PoliG Bertaccini
Oct 6, 1987·European Journal of Pharmacology·R RubinsteinS Cohen
Apr 28, 1983·Nature·J M ArrangJ C Schwartz
Jun 1, 1983·Urology·D Mitolo-ChieppaM R Carratú
Oct 1, 1994·Urology·P SeshadriA Morales
Aug 1, 1994·The Journal of Urology·M el-MansouryT C Theoharides
Dec 1, 1993·The Journal of Urology·S PaleaC Pietra
Mar 1, 1994·The Journal of Urology·P B Patra, D P Westfall
May 1, 1997·Urology·T C Theoharides, G R Sant
Aug 8, 2001·BJU International·P DasguptaP Dennis
Oct 12, 2001·Journal of Physiology, Paris·E PoliG Coruzzi
Dec 26, 2001·Molecular Pharmacology·Jordi Gomez-RamirezIsaac Blanco
May 7, 2002·The Journal of Urology·Deborah R EricksonSusan K Keay
Aug 13, 2002·Cell and Tissue Research·Jochen NeuhausWolfgang Dorschner
Feb 1, 2003·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·I Martínez-MirE Rubio-Gomis
Apr 12, 2003·Journal of Receptor and Signal Transduction Research·Timothy A EsbenshadeArthur A Hancock
Dec 31, 2003·Journal of Neuroimmunology·Theoharis C Theoharides, David E Cochrane
Mar 12, 2004·Urology·Karl-Erik Andersson
Dec 15, 2004·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Douglas G Tincello, Anthony C H Walker
Apr 13, 2006·World Journal of Urology·Jochen NeuhausWolfgang Dorschner
Citations
Oct 27, 2011·Advances in Urology·Jochen NeuhausJens-Uwe Stolzenburg
Oct 5, 2011·World Journal of Urology·Jochen NeuhausThilo Schwalenberg
Jun 20, 2015·Neurourology and Urodynamics·Qi-Xiang SongMargot S Damaser
Mar 9, 2019·Scientific Reports·Zane StrombergaChristian Moro