Culturing and electrophysiology of cells on NRCC patch-clamp chips.

Journal of Visualized Experiments : JoVE
Christophe PyGeoff Mealing

Abstract

Due to its exquisite sensitivity and the ability to monitor and control individual cells at the level of ion channels, patch-clamping is the gold standard of electrophysiology applied to disease models and pharmaceutical screens alike. The method traditionally involves gently contacting a cell with a glass pipette filled by a physiological solution in order to isolate a patch of the membrane under its apex. An electrode inserted in the pipette captures ion-channel activity within the membrane patch or, when ruptured, for the whole cell. In the last decade, patch-clamp chips have been proposed as an alternative: a suspended film separates the physiological medium from the culture medium, and an aperture microfabricated in the film replaces the apex of the pipette. Patch-clamp chips have been integrated in automated systems and commercialized for high-throughput screening. To increase throughput, they include the fluidic delivery of cells from suspension, their positioning on the aperture by suction, and automated routines to detect cell-to-probe seals and enter into whole cell mode. We have reported on the fabrication of a silicon patch-clamp chip with optimized impedance and orifice shape that permits the high-quality recording...Continue Reading

References

Mar 22, 2008·Nature Reviews. Drug Discovery·John DunlopRobert Arias
Mar 11, 2009·Frontiers in Bioscience (Landmark Edition)·Harold J Bell, Naweed I Syed
Sep 16, 2009·Biotechnology and Bioengineering·Anne CharrierGeoff Mealing
Aug 10, 2010·Biomedical Microdevices·Dolores MartinezGeoff Mealing
May 5, 2011·Journal of Neural Engineering·Marzia MartinaGeoffrey A R Mealing

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