PMID: 3762491Jan 1, 1986Paper

Culturing and extraction of Coprococcus comes, absorption of serumagglutinins by soluble fractions and relation between agglutinins and antibodies in sera of patients with Crohn's disease

Medical Microbiology and Immunology
M P HazenbergJ P van de Merwe

Abstract

Agglutinating antibodies to Coprococcus comes and three other obligately anaerobic coccoid rods from the intestinal flora are used in the diagnosis of Crohn's disease. Further studies on the pathogenetic role as well as the development of more sensitive and specific methods for detecting antibodies require extraction of the antigen fractions. Culturing methods to obtain C. comes with optimal antigen presentation and isolation of soluble antigen fractions were therefore developed. Hot water extraction of whole cells and subsequent removal of proteins with trichloroacetic acid provided a fraction that absorbed serum agglutinins, was useful for an enzyme-linked immunosorbent assay and induced agglutinating antibodies in rats.

References

Sep 1, 1971·The Journal of Hygiene·F Wensinck, J G Ruseler-van Embden
Jun 1, 1983·Antonie van Leeuwenhoek·J G Ruseler-van Embden, H C Both-Patoir
Aug 1, 1981·The Journal of Hygiene·F WensinckA M Schröder
Aug 1, 1981·The Journal of Hygiene·F Wensinck, J P Van de Merwe

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