Abstract
The ability to study HIV-1-specific cytotoxic T cell (CTL) clones in models in vitro or to expand them for immunotherapeutic use is limited by the technical difficulty of propagating these cells. The factors that determine the survival and proliferation of the cells are incompletely understood and could include cytokines provided from feeder cells or serum. We therefore investigated the effects of adding two cytokines reported to have effects on T cell proliferation and function, interleukin (IL)-7 and IL-15. Four HIV-1-specific clones derived from infected persons were cultured under standard conditions with IL-2 compared to IL-7 or IL-15 alone or in combination with IL-2. Proliferation and survival, as reflected by cell numbers after stimulation, were poorly supported by IL-7 or IL-15 alone, and these cytokines appeared to provide no additional benefit when added to IL-2. Similarly, these cytokines alone did not support the functional status of these cells as measured by chromium release assays with peptide-pulsed target cells. Addition of IL-7 or IL-15 to IL-2 did not augment function of the cells. These data suggest that supplementing CTL cultures with these cytokines does not provide improvement of cell growth or function.
References
Apr 1, 1992·European Journal of Immunology·M V WilesB A Imhof
Jan 1, 1992·International Immunology·M FabbiJ L Strominger
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·B D WalkerR T Schooley
Aug 1, 1995·Clinical and Experimental Immunology·G FerrariK J Weinhold
Apr 1, 1995·Nature Medicine·S KoenigC Carter
Apr 1, 1996·Immunity·Y TagayaT A Waldmann
Nov 1, 1996·Virology·F BuseyneY Rivière
May 1, 1997·Clinical and Experimental Immunology·J H KimD L Birx
Jul 1, 1997·Immunity·U von Freeden-JeffryR Murray
Oct 23, 1997·Proceedings of the National Academy of Sciences of the United States of America·O O YangB D Walker
Oct 23, 1997·Nature Medicine·S Bulfone-PausU Kunzendorf
Apr 30, 1998·Immunobiology·R V SorgD N Hart
Oct 13, 1998·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·P ShankarJ Lieberman
Feb 5, 1999·Science·J E SchmitzK A Reimann
Mar 17, 1999·The Journal of Experimental Medicine·X JinD D Ho
May 17, 2000·The Journal of Clinical Investigation·S J BrodieL Corey
Feb 27, 2001·Journal of Virology·M SchuttenA D Osterhaus
Jan 19, 2002·Journal of Virology·Otto O YangBruce D Walker
Jun 20, 2002·Journal of Virology·Livia Pedroza-MartinsChristel H Uittenbogaart
Sep 11, 2002·European Journal of Immunology·Carel A van BaalenRob A Gruters
Dec 5, 2002·Trends in Immunology·Victor Appay, Sarah L Rowland-Jones
Dec 5, 2002·Trends in Immunology·Debbie van BaarleFrank Miedema
Citations
Apr 19, 2005·Expert Opinion on Biological Therapy·L R Meireles-de-Souza, Robin J Shattock
Feb 21, 2007·Experimental Hematology·Christopher G Ramsborg, E Terry Papoutsakis
Oct 20, 2005·Clinical and Experimental Immunology·M MontesS Leyvraz