Culturing of mouse and human cells on soft substrates promote the expression of stem cell markers

Journal of Bioscience and Bioengineering
Sayaka HiguchiHideaki Fujita

Abstract

Substrate elasticity is a potent regulator of the cell state. Soft substrates have been shown to promote the homogeneous self-renewal of mouse embryonic stem cells through the down-regulation of cell-matrix tractions. We therefore investigated whether soft substrates promote the reprogramming of somatic cells into induced pluripotent stem (iPS) cells. After retroviral infection with five factors, Oct3/4, Klf4, Sox2, Lin28 and Nanog, mouse embryonic fibroblasts (MEFs) were cultured on several artificial substrates of varying elasticity and examined for the expression of pluripotency genes. When MEFs were cultured on soft (<0.1 kPa) polyacrylamide gels coated with gelatin, the expressions of Nanog and Oct3/4 genes were higher than in cells cultured on rigid plastic dishes (∼10(6) kPa). The same result was obtained at higher elasticity (0.5 kPa) for adult human dermal fibroblasts (HDFa). We also examined whether reprogramming could be enhanced on soft substrates without exogenous gene introduction, finding that cells cultured on soft substrates in the presence of chemicals known to promote cell reprogramming exhibited up-regulated stem cell markers. These results suggest that controlling the substrate stiffness can enhance the ini...Continue Reading

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Citations

Oct 18, 2015·Journal of Bioscience and Bioengineering·Hideki Mori, Masayuki Hara
Sep 17, 2016·Development, Growth & Differentiation·Sayaka HiguchiShohei Mitani
May 26, 2017·FEBS Letters·Hiroaki MachiyamaHideaki Fujita
Jul 8, 2017·Cell Adhesion & Migration·Wataru SugimotoKeiko Kawauchi
Jan 13, 2018·Tissue Engineering. Part B, Reviews·Xiaofang ChenYubo Fan
Feb 14, 2017·BioMed Research International·Takahiro EbataKeiko Kawauchi
Oct 3, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Joseph LongDeok-Ho Kim
Apr 24, 2018·Nucleic Acids Research·Roopali PradhanKundan Sengupta
Apr 11, 2019·Frontiers in Bioengineering and Biotechnology·Ryan J Hickey, Andrew E Pelling
Jul 17, 2020·The Journal of Cell Biology·Yanmei QiShaohua Li
May 16, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Hideki Mori, Masayuki Hara
Jul 15, 2021·Biology Open·Kazuko OkamotoHideaki Fujita

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