Cumene hydroperoxide debilitates macrophage physiology by inducing oxidative stress: possible protection by alpha-tocopherol

Chemico-biological Interactions
Gurpreet KaurMohammad Athar

Abstract

Macrophages, the major phagocytes of body, are largely dependent on membrane for their apposite functioning. Cum-OOH, a catalyst used in chemical and pharmaceutical industry, is a peroxidative agent, which may induce oxidative stress in macrophages hampering the integrity of their membrane. Alpha-tocopherol is known to protect the membrane from oxidative modulation and preserve its integrity. In the present study, we investigated the effect of Cum-OOH on physiology of macrophages and evaluated the protective effect of alpha-tocopherol against Cum-OOH-induced functional impairment. An in vitro exposure to 10-200 microM Cum-OOH altered redox balance of murine peritoneal macrophages and led to a severe physiological impairment. It markedly augmented the release of proinflammatory cytokines (tumor necrosis factor-alpha, interleukin-1beta and prostaglandin E(2)), lipopolysaccharide primed nitric oxide release and inducible nitric oxide synthase expression, and lysosomal hydrolases secretion. It mitigated respiratory burst and phagocytosis and intracellular killing of yeast (Saccharomyces cerevisiae). Mannose receptor, a major macrophage phagocytic receptor (also implicated in S. cerevisiae phagocytosis), exhibited a hampered recycli...Continue Reading

References

Jan 1, 1988·Free Radical Biology & Medicine·S N Chatterjee, S Agarwal
Jul 1, 1983·American Industrial Hygiene Association Journal·V CocheoG G Bombi
Oct 1, 1982·Analytical Biochemistry·L C GreenS R Tannenbaum
Feb 1, 1995·Free Radical Biology & Medicine·S M VroegopS E Buxser
Jan 1, 1996·Free Radical Biology & Medicine·V A KostyukG T Maslova
Jan 21, 2000·Free Radical Biology & Medicine·M J Raley, D J Loegering
Jun 1, 2000·Laboratory Investigation; a Journal of Technical Methods and Pathology·V Witko-SarsatL Halbwachs-Mecarelli
Aug 11, 2000·The American Journal of Medicine·B M Babior
Mar 12, 2004·Journal of Ethnopharmacology·Gurpreet KaurMohammad Athar

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