Cumulus cells surrounding oocytes with high developmental competence exhibit down-regulation of phosphoinositol 1,3 kinase/protein kinase B (PI3K/AKT) signalling genes involved in proliferation and survival

Human Reproduction
P G ArtiniItalian Society of embryology, Reproduction and Research (SIERR)

Abstract

Is the phosphoinositol 1,3-kinase/protein kinase B (PI3K/AKT) pathway expression profile in cumulus cells (CCs) a potential marker of oocyte competence and predictive of pregnancy outcome? Eleven genes (AKT1, ARHGEF7, BCL2L1, CCND1, E2F1, HRAS, KCNH2, PIK3C2A, SHC1, SOS1 and SPP1) in the PI3K/AKT pathway were significantly down-regulated in CCs from oocytes that went on to produce a pregnancy compared to CCs associated with a negative outcome. The PI3K/AKT pathway plays a pivotal role in the interdependence and continuous feedback between the oocyte and CCs. The expression analysis of 92 transcripts in the PI3K/AKT pathway in CCs from patients with negative or positive pregnancy outcome, after single embryo transfer, was performed. Mouse CCs target gene expression was conducted to associate the expression profile of PI3K/AKT pathway to oocyte developmental profile. Fifty-five good prognosis IVF patients who had been referred to IVF or intracytoplasmic sperm injection treatment for male-factor infertility or tubal disease were enroled. CCs from single cumulus-oocyte complexes (COCs) from 16 patients who underwent a single embryo transfer were analyzed. Twenty-five CD-1 mice were used to assess gene expression in CCs associated w...Continue Reading

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Citations

Sep 13, 2019·Molecular Reproduction and Development·Maria Cristina BudaniGian Mario Tiboni
Sep 2, 2020·Biomedicines·Fani KonstantinidouValentina Gatta
Dec 7, 2019·Reproductive Biology and Endocrinology : RB&E·Li-Hua ZhuNing-Yuan Zhang
Jan 23, 2021·Animals : an Open Access Journal From MDPI·Xiaosheng ZhangXiaofei Guo
May 8, 2020·Molecular and Cellular Endocrinology·Jiahui HuFenglei Chen
Mar 28, 2021·Archives of Gynecology and Obstetrics·Mojtaba MasoudiEnsieh Salehi

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Methods Mentioned

BETA
PCR
nucleotide exchange
nuclear translocation

Software Mentioned

DataAssist

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