Cumulus expansion and glucose utilisation by bovine cumulus-oocyte complexes during in vitro maturation: the influence of glucosamine and follicle-stimulating hormone

Reproduction : the Official Journal of the Society for the Study of Fertility
Melanie L Sutton-McDowallJeremy G Thompson

Abstract

Glucose is an important metabolite and its presence during in vitro oocyte maturation (IVM) can have profound effects on the oocyte's developmental capacity. We have demonstrated that glucose uptake increases over a 24 h IVM period, with most accounted for as l-lactate production. However, as maturation proceeds, l-lactate production remains constant, suggesting an alternative role for glucose metabolism. We hypothesised that in the latter stages of oocyte maturation, glucose not accounted for by l-lactate production is utilised for FSH-stimulated extracellular matrix (ECM) synthesis. To examine precursor utilisation for synthesis of ECM, bovine cumulus-oocyte complexes (COCs) were matured in +/- FSH and/or glucosamine (an alternative substrate of matrix components). Measurements included COC diameters, glucose consumption and l-lactate production in spent media and [U-(14)C]glucose incorporation into ECM. FSH significantly stimulated both diameter and glucose consumption during 20-24 h maturation compared with unstimulated complexes, although co-incubation with glucosamine and FSH decreased total glucose consumption 1.7-fold compared with FSH alone (P < 0.05). Furthermore, there was a linear relationship between glucose and l-...Continue Reading

References

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Jun 20, 2003·Reproduction : the Official Journal of the Society for the Study of Fertility·M L SuttonJ G Thompson

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Citations

Jul 20, 2011·Annals of Biomedical Engineering·A R ClarkJ G Thompson
Aug 1, 2008·Animal : an International Journal of Animal Bioscience·J L M R LeroyP E J Bols
May 11, 2010·Reproduction, Fertility, and Development·Cheryl J SchelbachJeremy G Thompson
May 4, 2010·Molecular Human Reproduction·Said AssouSamir Hamamah
Mar 16, 2006·The Journal of Reproduction and Development·Jeremy G Thompson
Mar 1, 2014·Animal Reproduction Science·Alma Álvarez-GuerreroNorma Moreno-Mendoza
Jan 22, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·Melanie L Sutton-McDowallJeremy G Thompson
Aug 22, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Sarah E HarrisHelen M Picton
Sep 27, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Kimberly A PreisDavid K Gardner
Jan 19, 2013·Reproduction, Fertility, and Development·Jason R Herrick
Dec 14, 2011·Computational and Mathematical Methods in Medicine·A R Clark, Y M Stokes
Sep 27, 2014·Bulletin of Experimental Biology and Medicine·I G SmetaninaA S Krivokharchenko
Sep 4, 2007·Molecular Human Reproduction·Maciej JózwikFrederick C Battaglia
Mar 9, 2011·Animal Reproduction Science·M J BertoldoC G Grupen
Sep 18, 2015·Domestic Animal Endocrinology·D BoruszewskaI Woclawek-Potocka
Jan 3, 2007·Animal Reproduction Science·Koji KimuraJeremy G Thompson
Nov 10, 2006·Theriogenology·Robert B Gilchrist, Jeremy G Thompson
Jan 4, 2012·Molecular Reproduction and Development·Nucharin SongsasenDavid E Wildt
Sep 7, 2016·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Ning ZhangDongmei Zhao

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