Curcumin Ameliorates Copper-Induced Neurotoxicity Through Inhibiting Oxidative Stress and Mitochondrial Apoptosis in SH-SY5Y Cells.

Neurochemical Research
Biao XiangShusheng Tang

Abstract

Impaired homeostasis of copper has been linked to different pathophysiological mechanisms in neurodegenerative diseases and oxidative injury has been proposed as the main mechanism. This study aims to use curcumin, a widely used antioxidative and anti-apoptotic agent, to exert the neuroprotective effect against copper in vitro and illuminate the underlying mechanism. The effect of curcumin was examined by using a cell counting kit-8 assay, flow cytometry, immunofluorescence, spectrophotometer, and western blot. Results revealed that after pretreatment with curcumin for 3 h, copper-induced toxicity and apoptosis show a significant decline. Further experiments showed that curcumin not only decreased the production of ROS and MDA but also increased the activities of the ROS scavenging enzymes SOD and CAT. Moreover, curcumin treatment alleviated the decrease in mitochondrial membrane potential and the nuclear translocation of cytochrome c induced by copper. The protein levels of pro-caspase 3, pro-caspase 9, and PARP1 were up-regulated and the Bax/Bcl-2 ratio was down-regulated in the presence of curcumin. Taken together, our study demonstrates that curcumin has neuroprotective properties against copper in SH-SY5Y cells and the pot...Continue Reading

References

Jan 1, 1985·Annual Review of Public Health·E J CalabreseC Sacco
Nov 17, 1983·Nature·J A TainerD C Richardson
Mar 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·P C WongJ D Gitlin
Jan 31, 2002·Proceedings of the National Academy of Sciences of the United States of America·Martin OttSten Orrenius
Jan 16, 2004·Gastroenterology·Jonathan D Gitlin
Apr 10, 2004·The Journal of Biological Chemistry·Alam Nur-E-KamalLeroy F Liu
Sep 22, 2006·Journal of Alzheimer's Disease : JAD·Michelangelo MancusoLuigi Murri
Jun 16, 2007·Advances in Experimental Medicine and Biology·Venugopal P Menon, Adluri Ram Sudheer
Nov 14, 2007·Journal of Applied Genetics·Alfredo Corona-RiveraJorge Roman Corona-Rivera
Oct 29, 2009·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Ya Hui HungRobert Alan Cherny
Feb 11, 2011·Environmental Geochemistry and Health·Lucyna Lewińska-PreisAndrzej Kita
Apr 2, 2013·Archives of Toxicology·Krishnendu SinhaParames C Sil
Aug 28, 2013·Proceedings of the National Academy of Sciences of the United States of America·Guiran XiaoBing Zhou
Jan 21, 2014·Progress in Neurobiology·Ivo F ScheiberRalf Dringen
Jan 28, 2014·Experimental Cell Research·Min Ok SongJonathan H Freedman
Feb 18, 2014·Toxicology and Applied Pharmacology·Zhuqin HuHongzhuan Chen
May 23, 2014·Current Biology : CB·Michael Schieber, Navdeep S Chandel
May 31, 2014·BioMed Research International·Soheil Zorofchian MoghadamtousiKeivan Zandi
Jan 27, 2015·Journal of Photochemistry and Photobiology. B, Biology·Fang HaoRutao Liu
Nov 4, 2015·Critical Reviews in Food Science and Nutrition·Betül Kocaadam, Nevin Şanlier
Jun 29, 2016·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·A P LanY Hu
Jul 7, 2016·Indian Journal of Clinical Biochemistry : IJCB·Emad A Hashish, Shimaa A Elgaml
Jul 21, 2016·Neurotoxicity Research·Ai-Ping LanYi Hu
Oct 28, 2016·Apoptosis : an International Journal on Programmed Cell Death·Rostyslav Horbay, Rostyslav Bilyy
Jul 30, 2017·The FEBS Journal·Aida Peña-Blanco, Ana J García-Sáez
Jun 20, 2018·Nature Chemical Biology·Jason Rihel
Oct 4, 2019·BioFactors·Hossein BagheriAmirhossein Sahebkar
Jan 24, 2020·Biochemical Pharmacology·Jun Wan ShinYoung-Joon Surh
Feb 7, 2020·Toxicology Reports·Amos O AbolajiEbenezer O Farombi

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Citations

May 4, 2021·FEBS Open Bio·Katiuska González-ArzolaMiguel A De la Rosa

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Methods Mentioned

BETA
MDA
Assay
confocal microscopy
nuclear translocation
protein assay
electrophoresis
flow cytometry

Software Mentioned

GraphPad Prism
NIH image - J
Image

Related Concepts

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis