PMID: 8950193Nov 15, 1996Paper

Curcumin, an antioxidant and anti-tumor promoter, induces apoptosis in human leukemia cells

Biochimica Et Biophysica Acta
M L KuoJ T Lin

Abstract

Curcumin, widely used as a spice and coloring agent in food, possesses potent antioxidant, anti-inflammatory and anti-tumor promoting activities. In the present study, curcumin was found to induce apoptotic cell death in promyelocytic leukemia HL-60 cells at concentrations as low as 3.5 micrograms/ml. The apoptosis-inducing activity of curcumin appeared in a dose- and time-dependent manner. Flow cytometric analysis showed that the hypodiploid DNA peak of propidium iodide-stained nuclei appeared at 4 h after 7 micrograms/ml curcumin treatment. The apoptosis-inducing activity of curcumin was not affected by cycloheximide, actinomycin D, EGTA, W7 (calmodulin inhibitor), sodium orthovanadate, or genistein. By contrast, an endonuclease inhibitor ZnSO4 and proteinase inhibitor N-tosyl-L-lysine chloro-methyl ketone (TLCK) could markedly abrogate apoptosis induced by curcumin, whereas 12-O-tetradecanoylphorbol-13-acetate (TPA) had a partial effect. The antioxidants, N-acetyl-L-cysteine (NAC), L-ascorbic acid, alpha-tocopherol, catalase and superoxide dismutase, all effectively prevented curcumin-induced apoptosis. This result suggested that curcumin-induced cell death was mediated by reactive oxygen species. Immunoblot analysis showed ...Continue Reading

References

Jun 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·T S HuangJ K Lin
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·Y Tsujimoto
Oct 22, 1993·Cell·D M HockenberyS J Korsmeyer

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Citations

Nov 24, 2006·Archives of Pharmacal Research·Songyot AnuchapreedaPornngarm Limtrakul
Feb 24, 2006·Archives of Pharmacal Research·Songyot AnuchapreedaPrasit Chanarat
Jul 16, 2005·Cancer Chemotherapy and Pharmacology·Wanida ChearwaePornngarm Limtrakul
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Dec 12, 2007·Investigational New Drugs·Denise K WaltersBruno Fuchs
Sep 25, 2012·Journal of Physiology and Biochemistry·Aysegul CortTomris Ozben
Mar 17, 2004·Free Radical Biology & Medicine·Heidi LigeretDidier Morin
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Apr 3, 2001·European Journal of Pharmacology·C Y ChuT H Tseng
Oct 19, 2000·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·S E ChuangM L Kuo
May 1, 2004·Critical Reviews in Food Science and Nutrition·B JoeB R Lokesh
Mar 29, 2008·Antioxidants & Redox Signaling·Alexios S Strimpakos, Ricky A Sharma
Sep 8, 2007·Antioxidants & Redox Signaling·Cheng LiRoland Stocker
Feb 24, 2006·Acta Pharmacologica Sinica·Songyot AnuchapreedaPornngarm Limtrakul
Aug 29, 2007·Acta Pharmacologica Sinica·Lynne M HowellsMargaret M Manson
May 28, 2009·Antimicrobial Agents and Chemotherapy·Monika SharmaRajendra Prasad
Jul 3, 2008·Cancer Research·Gayathri ChadalapakaStephen Safe
Sep 6, 2011·Molecular Cancer Therapeutics·Dharmalingam SubramaniamShrikant Anant
Dec 15, 2010·International Journal of Molecular Sciences·Chia-Chi ChenWen-Hsiung Chan

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