Curcumin Analog DK1 Induces Apoptosis in Human Osteosarcoma Cells In Vitro through Mitochondria-Dependent Signaling Pathway

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Muhammad Nazirul Mubin AzizNoorjahan Banu Alitheen

Abstract

Osteosarcoma is one of the primary malignant bone tumors that confer low survival rates for patients even with intensive regime treatments. Therefore, discovery of novel anti-osteosarcoma drugs derived from natural products that are not harmful to the normal cells remains crucial. Curcumin is one of the natural substances that have been extensively studied due to its anti-cancer properties and is pharmacologically safe considering its ubiquitous consumption for centuries. However, curcumin suffers from a poor circulating bioavailability, which has led to the development of a chemically synthesized curcuminoid analog, namely (Z)-3-hydroxy-1-(2-hydroxyphenyl)-3-phenylprop-2-en-1-one (DK1). In this study, the cytotoxic effects of the curcumin analog DK1 was investigated in both U-2OS and MG-63 osteosarcoma cell lines using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and cell death was microscopically examined via acridine orange/propidium iodide (AO/PI) double staining. Flow cytometer analysis including Annexin V/Fluorescein isothiocyanate (FITC), cell cycle analysis and JC-1 were adapted to determine the mode of cell death. Subsequently in order to determine the mechanism of cell death, quantitative p...Continue Reading

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Sep 21, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Muhammad Yusran Abdul AzizNoorjahan Banu Alitheen
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Citations

Apr 12, 2018·International Journal of Molecular Sciences·Yazmin HussinNoorjahan Banu Alitheen
Jun 9, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Qingbin CuiZhe-Sheng Chen
Oct 24, 2018·Cancer Cell International·Babak OtoukeshMaria Kaghazian
Mar 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nurul Fattin Che RahimNoorjahan Banu Alitheen
Aug 13, 2021·Immunotherapy·Jorge H Tabares-GuevaraJuan C Hernandez

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Methods Mentioned

BETA
X-ray
profiler
chemical modification
ELISA
Assay
flow cytometry
PCR

Software Mentioned

EcoStudy
SPSS

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