Curcumin Attenuates gp120-Induced Microglial Inflammation by Inhibiting Autophagy via the PI3K Pathway

Cellular and Molecular Neurobiology
Guiling ChenJun Dong

Abstract

Microglial inflammation plays an essential role in the pathogenesis of HIV-associated neurocognitive disorders. A previous study indicated that curcumin relieved microglial inflammatory responses. However, the mechanism of this process remained unclear. Autophagy is a lysosome-mediated cell content-dependent degradation pathway, and uncontrolled autophagy leads to enhanced inflammation. The role of autophagy in curcumin-attenuating BV2 cell inflammation caused by gp120 was investigated with or without pretreatment with the autophagy inhibitor 3-MA and blockers of NF-κB, IKK, AKT, and PI3K, and we then detected the production of the inflammatory mediators monocyte chemoattractant protein-1 (MCP-1) and IL17 using ELISA, and autophagy markers ATG5 and LC3 II by Western Blot. The autophagic flux was observed by transuding mRFP-GFP-LC3 adenovirus. The effect of the blockers on gp120-induced BV2 cells was examined by the expression of p-AKT, p-IKK, NF-κB, and p65 in the nuclei and LC3 II and ATG5. gp120 promoted the expression of MCP-1 and IL-17, enhanced autophagic flux, and up-regulated the expression of LC3 II and ATG5, while the autophagy inhibitor 3-MA down-regulated the phenomena above. Curcumin has similar effects with 3-MA, i...Continue Reading

References

Jan 30, 2003·Annals of Medicine·Peter T NelsonEhud Lavi
Jan 5, 2005·Nature Reviews. Immunology·Francisco González-Scarano, Julio Martín-García
Jun 24, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Gamal BadrYolande Richard
May 6, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ricky CheungRonald G Collman
Sep 24, 2013·Journal of Cardiovascular Pharmacology and Therapeutics·Yunxiao SongHaichen Zhang
Sep 24, 2013·Journal of Cardiovascular Pharmacology and Therapeutics·Keping YangHong Jiang
Jun 5, 2014·AIDS Research and Therapy·Vasudev R RaoVinayaka R Prasad
Jun 9, 2014·Lancet·Gary MaartensSharon R Lewin
Dec 3, 2014·Brain, Behavior, and Immunity·Suzi Hong, William A Banks
Feb 11, 2015·Experimental and Molecular Pathology·Zhao YangBangqing Yuan
Feb 13, 2015·Molecular Medicine Reports·Zhouqing HuangWeijian Huang
Mar 17, 2015·Endocrine, Metabolic & Immune Disorders Drug Targets·Atsuko Deguchi
Apr 18, 2015·Free Radical Biology & Medicine·Asta JurkuvenaiteSadis Matalon
Jun 13, 2015·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Shatadal GhoshParames C Sil
Nov 27, 2015·Acta Biochimica Et Biophysica Sinica·Feng XieLinxi Chen
Jan 23, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Özge BerrakNarçin Palavan-Ünsal
Apr 22, 2016·Biotechnology Letters·Sridharan JeyamohanAntony Joseph Velanganni Arockiam
May 22, 2016·International Immunopharmacology·Antonia CianciulliMaria Antonietta Panaro
Jul 22, 2016·Oncotarget·Hadjer AbdelouahabFawzia Louache

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Citations

Apr 6, 2019·Neurotoxicity Research·Faezeh GhasemiAmirhossein Sahebkar
Nov 12, 2019·Frontiers in Cellular and Infection Microbiology·Clementine WalletChristian Schwartz
Jan 18, 2020·Biomolecules·Antonia CianciulliMaria Antonietta Panaro
Mar 19, 2020·Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy·Pingping ZhangDongmei Gan
Nov 28, 2018·International Journal of Molecular Sciences·Karina Sánchez-AlegríaClorinda Arias
Jul 12, 2020·Fluids and Barriers of the CNS·Amila OmeragicReina Bendayan
May 22, 2020·Molecular Neurobiology·Kevin M BudgeFayez F Safadi
Apr 2, 2021·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Xueqin YanJun Dong

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Methods Mentioned

BETA
ELISA

Key Resources (RRID) Mentioned

CVCL_0182

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