Curcumin attenuates Mancozeb-induced toxicity in rat thymocytes through mitochondrial survival pathway

Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
Voja PavlovicJelena Jovanovic

Abstract

The widely used fungicide Mancozeb (Man) has been shown to cause genotoxic effects in rodents and toxicological manifestations in different cells, mainly by altering the antioxidant defense in cells. On the other hand, curcumin (Cur), a natural phenolic compound, is thought to possess anti-inflammatory and antioxidant properties. Here, we investigated the possible protective role of Cur on Man-induced toxicity in rat thymocytes and potential mechanism involved. Rat thymocytes were treated with Man(0.01 μg/ml) and/or increasing Cur(0.3, 1, 3 μM) concentrations and levels of cell viability, apoptosis, mitochondrial membrane potential (MMP),Bcl-2, Bax protein expression, caspase-3 and -9 activity and p38 MAPK signaling involvement were examined. Cells treated with Man displayed increased cell toxicity, hypodiploid cells, caspase-3 and -9 activity, Bax protein expression, followed with decreased MMP and Bcl-2 protein expression. Inhibition of p38 MAPK signaling pathway markedly reduced apoptosis rate and caspase-3 activity in thymocytes exposed to Man. Application of increasing Cur (1, 3 μM) concentrations resulted with significantly reduced cytotoxicity, apoptosis, caspase-3, -9 activity, Bax protein expression, together with incr...Continue Reading

References

Jul 26, 2000·The Journal of Cell Biology·S GhatanR S Morrison
Jan 12, 2002·The Journal of Biological Chemistry·Rio HoriMakoto Suematsu
Nov 5, 2003·Journal of Cellular and Molecular Medicine·Alexandrina Burlacu
Jan 28, 2004·Cell·Nika N Danial, Stanley J Korsmeyer
May 17, 2005·Toxicology and Applied Pharmacology·Emanuela CorsiniClaudio Colosio
Jul 12, 2005·Toxicology and Applied Pharmacology·Gabriella CalvielloPaola Palozza
Mar 16, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jun CaoLai-Fu Zhong
Oct 3, 2006·Neurotoxicology·Laura J Leiphon, Matthew J Picklo
Mar 23, 2011·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Kazuki KoizumiYasuo Oyama
Feb 1, 2012·Life Sciences·Amit Kumar SrivastavaYogeshwer Shukla
Mar 1, 2012·Toxicology and Applied Pharmacology·Rita ParoSandra Cecconi
May 15, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Voja PavlovicGordana Stojanovic
Jul 31, 2013·BMC Complementary and Alternative Medicine·Manish K SinghSanjay Khattri
Mar 7, 2014·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Ning KangXiu-Mei Gao
Jun 11, 2014·Pharmacognosy Magazine·Nayereh ShafaghatiSeyed Jalal Hosseinimehr
Dec 6, 2014·Journal of Clinical and Diagnostic Research : JCDR·Sai Krishna BorraLittle Mahendra
Dec 30, 2014·Toxicology and Applied Pharmacology·Kahkashan Rashid, Parames C Sil
Sep 27, 2015·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Roberto IorioSandra Cecconi

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Citations

May 17, 2018·Toxicological Sciences : an Official Journal of the Society of Toxicology·Colin C AndersonJames R Roede
Jul 25, 2018·Toxicology and Industrial Health·Mohaddeseh Mohammadi-SardooBagher Amirheidari
Aug 3, 2019·Ecotoxicology and Environmental Safety·Taghred M SaberHeba M Abdel-Ghany
Apr 10, 2018·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Jingjing LuZhigang Zhang
Oct 14, 2021·Toxicology and Industrial Health·Mohaddeseh Mohammadi-SardooBagher Amirheidari

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