Curcumin Induces Neural Differentiation of Human Pluripotent Embryonal Carcinoma Cells through the Activation of Autophagy

BioMed Research International
Nudjanad HeebkaewParinya Noisa

Abstract

Curcumin is a natural polyphenolic compound, isolated from Curcuma longa, and is an important ingredient of Asian foods. Curcumin has revealed its strong activities of anti-inflammatory, antioxidant, and anticancer. The efficient amount of curcumin could induce differentiation of stem cells and promoted the differentiation of glioma-initiating cells; however, the mechanisms underlying neural induction of curcumin have not yet been revealed. In this study, neural-inducing ability of curcumin was explored by using human pluripotent embryonal carcinoma cells, NTERA2 cells. The cells were induced toward neural lineage with curcumin and were compared with a standard neutralizing agent (retinoic acid). It was found that, after 14 days of the induction by curcumin, NTERA2 cells showed neuronal morphology and expressed neural-specific genes, including NeuroD, TUJ1, and PAX6. Importantly, curcumin activated neurogenesis of NTERA2 cells via the activation of autophagy, since autophagy-related genes, such as LC3, LAMP1, and ATG5, were upregulated along with the expression of neural genes. The inhibition of autophagy by chloroquine suppressed both autophagy and neural differentiation, highlighting the positive role of autophagy during neur...Continue Reading

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Citations

Jul 7, 2019·International Journal of Molecular Sciences·Fiona LimanaqiFrancesco Fornai
Jan 25, 2020·Biotechnology and Applied Biochemistry·Shadi HeidariFatemeh Kalalinia
May 26, 2020·Seminars in Cancer Biology·Srimanta PatraSujit K Bhutia
Jun 3, 2021·International Journal of Molecular Sciences·Pablo BonillaVictoria Moreno-Manzano
Aug 2, 2021·Cellular and Molecular Life Sciences : CMLS·Mariana MedeirosMaría Sol Brassesco

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Methods Mentioned

BETA
PCR
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Software Mentioned

SPSS

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