Curcumin inhibits autocrine growth hormone-mediated invasion and metastasis by targeting NF-κB signaling and polyamine metabolism in breast cancer cells

Amino Acids
Ajda Coker-GurkanNarçin Palavan-Unsal

Abstract

Curcumin is assumed to be a plant-derived therapeutic drug that triggers apoptotic cell death in vitro and in vivo by affecting different molecular targets such as NF-κB. Phase I/II trial of curcumin alone or with chemotherapeutic drugs has been accomplished in pancreatic, colon, prostate and breast cancer cases. Recently, autocrine growth hormone (GH) signaling-induced cell growth, metastasis and drug resistance have been demonstrated in breast cancer. In this study, our aim was to investigate the potential therapeutic effect of curcumin by evaluating the molecular machinery of curcumin-triggered apoptotic cell death via focusing on NF-κB signaling and polyamine (PA) metabolism in autocrine GH-expressing MCF-7, MDA-MB-453 and MDA-MB-231 breast cancer cells. For this purpose, a pcDNA3.1 (+) vector with a GH gene insert was transfected by a liposomal agent in all breast cancer cells and then selection was conducted in neomycin (G418) included media. Autocrine GH-induced curcumin resistance was overcome in a dose-dependent manner and curcumin inhibited cell proliferation, invasion-metastasis and phosphorylation of p65 (Ser536), and thereby partly prevented its DNA binding activity in breast cancer cells. Moreover, curcumin induce...Continue Reading

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Citations

Feb 23, 2020·Seminars in Cancer Biology·Sajad FakhriAnupam Bishayee
Sep 1, 2021·European Journal of Pharmacology·Qi-Zhang LiYan Ji

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Methods Mentioned

BETA
PCR
electrophoresis
transfection
ELISA
Assay
light microscopy
protein assay
FACS
flow cytometry
scraping

Software Mentioned

GraphPad
ProteoJET
BD Accuri C6
C6
GraphPad Prism
Image J

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