Curcumin-mediated demethylation of the proximal promoter CpG island enhances the KLF4 recruitment that leads to increased expression of p21Cip1 in vitro

Journal of Cellular Biochemistry
Biji ChatterjeeSantosh R Kanade

Abstract

Curcumin, the active component of the spice turmeric, induce global DNA hypomethylation as it has been shown to inhibit DNA methyltransferases. It promotes cell death in cancer cells by arresting in the G1 phase. It was explained to cause increased expression of cell cycle regulator, p21 (WAF1/Cip1); however, the mechanism remains not clear. The p21 promoter harvests a CpG island (CGI) in the proximal region enriched with CG dinucleotide clusters with Kruppel-like factor 4 (KLF4) transcription factor binding site. We probed the p21 promoter CGI (spanning from -135 to +12, respective to the transcription start site) to detect alterations in cytosine methylation level in response to curcumin exposure in four different human cancer cell lines: A431, A549, MCF7, and HeLa. We observed curcumin (20 µM) treatment significantly increased the expression of p21, and the promoter CGI was demethylated in a dose-dependent manner. The curcumin significantly raised the level KLF4 and enhanced the p21 promoter occupancy by KLF4. From our results we hypothesize that curcumin-mediated demethylation of the p21 proximal promoter and increased KLF4 expression as well as its binding to its proximal promoter could serve as a mechanism that could be h...Continue Reading

References

Jun 5, 2002·World Journal of Gastroenterology : WJG·Jing-Yuan Fang, You-Yong Lu
Nov 9, 2002·Bioinformatics·Long-Cheng Li, Rajvir Dahiya
May 30, 2003·Molecular and Cellular Biology·Wei-Guo ZhuGregory A Otterson
May 19, 2005·Cancer Research·Andrei L Gartel, Senthil K Radhakrishnan
Nov 9, 2005·Prostate Cancer and Prostatic Diseases·S R J BottM Williamson
Dec 24, 2005·Nature Reviews. Cancer·Benjamin D Rowland, Daniel S Peeper
Nov 7, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Masashi KanaiKeping Xie
Jul 8, 2008·Acta Biochimica Et Biophysica Sinica·Paul M Evans, Chunming Liu
Dec 30, 2008·Bioorganic & Medicinal Chemistry Letters·Zhongfa LiuKenneth K Chan
Mar 12, 2009·Biochemical and Biophysical Research Communications·Hwee Hong Tan, Alan George Porter
May 15, 2009·Nature Reviews. Cancer·Tarek Abbas, Anindya Dutta
Sep 10, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Wenxian HuBingliang Fang
Sep 16, 2009·Carcinogenesis·Shikhar SharmaPeter A Jones
Oct 21, 2010·Physiological Reviews·Beth B McConnell, Vincent W Yang
Feb 3, 2012·Molecular and Cellular Biochemistry·Gaurav ParasharNeena Capalash
Sep 8, 2012·Advances in Experimental Medicine and Biology·Melanie Ehrlich, Michelle Lacey
Nov 20, 2012·Current Opinion in Oncology·Noel A Warfel, Wafik S El-Deiry
Sep 26, 2013·Nature Reviews. Cancer·Marie-Pier TetreaultJonathan P Katz
Apr 10, 2014·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Yongxia ZhuLuoting Yu
Jul 1, 2014·Archives of Pathology & Laboratory Medicine·José Manuel Naranjo GómezJose Javier Gómez Roman
May 20, 2015·Asian Pacific Journal of Cancer Prevention : APJCP·Bo DingChun-Ling Wang
Nov 4, 2015·Critical Reviews in Food Science and Nutrition·Betül Kocaadam, Nevin Şanlier
Apr 27, 2016·Chinese Journal of Natural Medicines·Peng WuChao Wu
May 20, 2016·Cold Spring Harbor Perspectives in Biology·Stephen B Baylin, Peter A Jones
Jun 10, 2016·Critical Reviews in Eukaryotic Gene Expression·Amna ParveenWhang Wan Kyunn
Oct 21, 2016·Molecular Oncology·Saroor A Patel, Sakari Vanharanta
Oct 30, 2016·Anticancer Research·Y I DengRamin Rohanizadeh
Dec 14, 2016·Biochemical Pharmacology·Dany PechalrieuPaola B Arimondo
Jan 28, 2017·Biomarker Research·Wu Zhang, Jie Xu
Feb 23, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Siying ZhouJinhai Tang
Apr 25, 2017·Journal of Experimental Pharmacology·Abir Kumar PandaGaurisankar Sa
May 6, 2017·Science·Timothy R Hughes, Samuel A Lambert
Jul 21, 2017·Journal of Experimental & Clinical Cancer Research : CR·Urmila BanikNor Hayati Othman

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Jan 3, 2021·Digestive Diseases and Sciences·Alexandra M KaneVicki L J Whitehall
Feb 24, 2020·Seminars in Cancer Biology·Wenhao Weng, Ajay Goel
Dec 17, 2020·The Journal of Nutritional Biochemistry·Lei FangBa Xuan Hoang
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