Curcumin protects neural cells against ischemic injury in N2a cells and mouse brain with ischemic stroke

Brain and Behavior
Cai-Jun XieRui-Cong Chen

Abstract

Curcumin, a natural antioxidant isolated from Curcuma longa, has been reported to exert neuroprotective effect in animal models of ischemic stroke. However, the underlying mechanism is still not fully understood. The purpose of this study was to investigate the effect of curcumin treatment on neuronal apoptosis in the periinfarct cortex after cerebral ischemia/reperfusion (I/R) injury and in mouse N2a cells after oxygen-glucose deprivation/reoxygenation (OGD/R) injury and its underlying mechanism. The cerebral I/R injury was established by 1-hr middle cerebral artery occlusion (MCAO) and reperfusion in mice. Infarct volume was determined by TTC staining, and neurological score was evaluated by mNSS. Cell morphology in the ischemic boundary zone were detected by HE staining. The number and apoptotic rate of neurons in ischemic boundary zone were assayed by immunohistochemistry and TUNEL, respectively. Mouse neuroblastoma N2a cells were subjected to OGD/R. Cell viability was assessed with CCK-8. The mitochondrial membrane potential was measured using JC-1 staining. The expression of Bax, Bcl-2, and caspase-3 was detected using Western blotting. Besides, cellular distribution of Bax was determined by immunofluorescence assays. Cur...Continue Reading

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Citations

Mar 6, 2019·Acta neurologica Belgica·Kun FuYunan Bai
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May 23, 2020·Nutritional Neuroscience·Raffaella Adami, Daniele Bottai
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Methods Mentioned

BETA
confocal microscopy

Software Mentioned

SPSS
Image J

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