Curcumin sensitized the antitumour effects of irradiation in promoting apoptosis of oesophageal squamous-cell carcinoma through NF-κB signalling pathway

The Journal of Pharmacy and Pharmacology
Guiping LiuMingjun Li

Abstract

To investigate the potential synergistic effect of curcumin with irradiation (IR) in oesophageal squamous-cell carcinoma (ESCC) and elucidate the underlying molecular mechanisms. The ESCC cell lines were established from clinical samples. Cell apoptosis post-treatment was stained by Annexin V/PI staining and analysed by flow cytometry. Cells survived IR was evaluated with clonogenic assay. Xenograft tumour model was established by subcutaneous inoculation, and tumour progression was monitored. The NF-κB pathway was characterized by immunoblotting. Curcumin enhanced the pro-apoptotic effect of IR in ESCC cells. Pretreatment with curcumin significantly sensitized ESCC cells to IR in a dose-dependent manner. Coadministration with curcumin remarkably extended the median survival time of ESCC xenograft mice while exposed to IR therapy. The xenograft tumour progression was significantly suppressed as well. Mechanistically, curcumin treatment was demonstrated to efficiently inhibited NF-κB signalling. We have well-recapitulated the pathological properties and therapeutic response of ESCC with established cell lines derived from clinical samples. We further demonstrated the significantly synergistic effect of curcumin on IR-elicited ce...Continue Reading

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Feb 3, 2019·Journal of Cellular Physiology·Kah Keng WongSiti Muhamad Nur Husna
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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis