Curcumol inhibits colorectal cancer proliferation by targeting miR-21 and modulated PTEN/PI3K/Akt pathways

Life Sciences
Haowei LiuXu Chen

Abstract

The purpose of this study was to demonstrate how curcumol affected the expression of miR-21 and whether its effects on miR-21 was associated with the activation of PTEN/PI3K/Akt pathways in CRC cells. MTT and xenograft assay were used to examine how curcumol inhibits colorectal cancer (CRC) cells' growth. Q-PCR and western blot analysis were employed to test the role of miR-21 in the inhibition of curcumol on proliferation and PTEN/PI3K/Akt pathways of CRC cells. We found that curcumol effectively inhibited CRC cells from proliferating via the PTEN/PI3K/Akt pathways and reduced expression of miR-21 both in vitro and in vivo. miR-21 mimics were found to decrease the protein level of PTEN and increase the expression of PI3K, phospho-Akt (p-Akt) and NF-κB, while miR-21 sponge (miR-21-SP) enhanced the expression of PTEN and reduced the activity of PI3K, Akt and NF-κB. Furthermore, miR-21-SP strengthened the role of curcumol in up-regulating PTEN and inhibiting PI3K/Akt pathways, but miR-21 reversed the effect of curcumol on the PTEN/PI3K/Akt pathways. Our research demonstrated that curcumol reduced the proliferation of CRC cells through PTEN/PI3K/Akt by targeting miR-21 and miR-21 could be a target molecule of curcumol for CRC trea...Continue Reading

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Citations

Apr 15, 2020·Nutrition and Cancer·Sheema HashemAjaz A Bhat
Feb 23, 2021·Frontiers in Oncology·Rilei JiangJiang Wu
Mar 16, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yi ChenWan Liao
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Dec 22, 2020·Biochemical Pharmacology·Mir S AdilPayaningal R Somanath
Jul 9, 2021·Journal of Asian Natural Products Research·Xiang-Wei MengXing-Xian Zhang

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