Curdlan-Conjugated PLGA Nanoparticles Possess Macrophage Stimulant Activity and Drug Delivery Capabilities

Pharmaceutical Research
Matshawandile TukululaAdmire Dube

Abstract

There is significant interest in the application of nanoparticles to deliver immunostimulatory signals to cells. We hypothesized that curdlan (immune stimulating polymer) could be conjugated to PLGA and nanoparticles from this copolymer would possess immunostimulatory activity, be non-cytotoxic and function as an effective sustained drug release system. Carbodiimide chemistry was employed to conjugate curdlan to PLGA. The conjugate (C-PLGA) was characterized using (1)H and (13)C NMR, FTIR, DSC and TGA. Nanoparticles were synthesized using an emulsion-solvent evaporation technique. Immunostimulatory activity was characterized in THP-1 derived macrophages. MTT assay and real-time impedance measurements were used to characterize polymer and nanoparticle toxicity and uptake in macrophages. Drug delivery capability was assessed across Caco-2 cells using rifampicin as a model drug. Spectral characterization confirmed successful synthesis of C-PLGA. C-PLGA nanoparticles enhanced phosphorylated ERK production in macrophages indicating cell stimulation. Nanoparticles provided slow release of rifampicin across Caco-2 cells. Polymers but not nanoparticles altered the adhesion profiles of the macrophages. Impedance measurements suggested C...Continue Reading

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Citations

Feb 4, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Agata Przekora, Grazyna Ginalska
Oct 20, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Agata PrzekoraGrazyna Ginalska
Apr 28, 2016·Advanced Drug Delivery Reviews·Ana BeloquiVéronique Préat
Jul 11, 2019·Applied Microbiology and Biotechnology·Mahmood Alizadeh-SaniArezou Khezerlou
Oct 6, 2020·Frontiers in Cellular and Infection Microbiology·Brenda KischkelCarlos P Taborda

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