Current applications of liquid chromatography/mass spectrometry in pharmaceutical discovery after a decade of innovation

Annual Review of Analytical Chemistry
Bradley L AckermannAjai K Chaudhary

Abstract

Current drug discovery involves a highly iterative process pertaining to three core disciplines: biology, chemistry, and drug disposition. For most pharmaceutical companies the path to a drug candidate comprises similar stages: target identification, biological screening, lead generation, lead optimization, and candidate selection. Over the past decade, the overall efficiency of drug discovery has been greatly improved by a single instrumental technique, liquid chromatography/mass spectrometry (LC/MS). Transformed by the commercial introduction of the atmospheric pressure ionization interface in the mid-1990s, LC/MS has expanded into almost every area of drug discovery. In many cases, drug discovery workflow has been changed owing to vastly improved efficiency. This review examines recent trends for these three core disciplines and presents seminal examples where LC/MS has altered the current approach to drug discovery.

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Citations

Jul 20, 2010·Annual Review of Analytical Chemistry·Suzanne Bell
Jan 30, 2014·Bioanalysis·Katarzyna Pawlak, Wioletta Jakubczak
Sep 17, 2014·The Journal of Cell Biology·Fred D MastJohn D Aitchison
Feb 19, 2011·The Kaohsiung Journal of Medical Sciences·Shyh-Horng Chiou, Chi-Yue Wu
Aug 20, 2009·European Journal of Lipid Science and Technology : EJLST·Xianlin Han, Xuntian Jiang
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Jul 11, 2014·Analytical Biochemistry·Wenyu YuPeter J Brown
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Feb 4, 2011·Bioanalytical Reviews·Tobias Kind, Oliver Fiehn

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Methods Mentioned

BETA
gene knockout
in
electrophoresis
glycosylation
nuclear magnetic resonance
PCA
electron capture
affinity capture
scintillation proximity assay
ELISA

Software Mentioned

SILAC
MASCOT
HTOS
MUX
AQUA
LG
MudPIT
SEQUEST
Tandem

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