Current knowledge of the Escherichia coli phosphoenolpyruvate-carbohydrate phosphotransferase system: peculiarities of regulation and impact on growth and product formation

Applied Microbiology and Biotechnology
Adelfo EscalanteFrancisco Bolívar

Abstract

In Escherichia coli, the phosphoenolpyruvate-carbohydrate phosphotransferase system (PTS) is responsible for the transport and phosphorylation of sugars, such as glucose. PTS activity has a crucial role in the global signaling system that controls the preferential consumption of glucose over other carbon sources. When the cell is exposed to carbohydrate mixtures, the PTS prevents the expression of catabolic genes and activity of non-PTS sugars transport systems by carbon catabolite repression (CCR). This process defines some metabolic and physiological constraints that must be considered during the development of production strains. In this review, we summarize the importance of the PTS in controlling and influencing both PTS and non-PTS sugar transport processes as well as the mechanisms of transcriptional control involved in the expression of catabolic genes of non-PTS sugars in E. coli. We discuss three main approaches applied efficiently to avoid these constraints resulting in obtaining PTS(-) glc(+) mutants useful for production purposes: (1) adaptive selection in chemostat culture system of PTS(-) mutants, resulting in the selection of strains that recovered the ability to grow in glucose, along with the simultaneous cons...Continue Reading

References

Jan 1, 1976·Annual Review of Microbiology·E C Lin
Oct 5, 1988·Journal of Molecular Biology·M Yamada, M H Saier
Jul 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J Adler, W Epstein
Aug 14, 2001·Applied Microbiology and Biotechnology·N N NicholsR J Bothast
Oct 18, 2001·Journal of the American Chemical Society·D R KnopJ W Frost
Nov 28, 2001·Environmental Microbiology·T Ferenci
Apr 6, 2002·Current Opinion in Microbiology·Jacqueline Plumbridge
May 25, 2002·Journal of Bacteriology·Debra W JacksonTony Romeo
Oct 31, 2002·Journal of Industrial Microbiology & Biotechnology·B S DienR J Bothast
Jun 7, 2003·Biotechnology Progress·Sunil S ChandranJ W Frost
Dec 4, 2003·Metabolic Engineering·Marco KrämerLeon Raeven
Jun 1, 2004·The Journal of Biological Chemistry·Byoung-Mo KooYeong-Jae Seok
Sep 24, 2005·Journal of Molecular Microbiology and Biotechnology·Noemí FloresFrancisco Bolívar
Jan 13, 2006·The Journal of Biological Chemistry·Young-Ha ParkAlan Peterkofsky
Aug 4, 2006·Journal of Bacteriology·Carlos BalsalobreBernt Eric Uhlin
Dec 13, 2006·Microbiology and Molecular Biology Reviews : MMBR·Josef DeutscherPieter W Postma
Mar 27, 2007·Current Opinion in Microbiology·Carin K Vanderpool
Sep 21, 2007·Microbial Cell Factories·José Luis Báez-ViverosGuillermo Gosset
Mar 6, 2008·Proceedings of the National Academy of Sciences of the United States of America·Tae-Wook NamSun-Shin Cha
Mar 25, 2008·Current Opinion in Microbiology·Josef Deutscher
May 27, 2008·The Journal of Antibiotics·Kunihiko NishinoAkihito Yamaguchi
Jul 17, 2008·Nature Reviews. Microbiology·Boris Görke, Jörg Stülke

❮ Previous
Next ❯

Citations

Aug 17, 2012·Applied Microbiology and Biotechnology·Jinlei TangXueli Zhang
Jun 7, 2014·Bioresource Technology·Xianzhong ChenZhengxiang Wang
Sep 10, 2014·Microbial Cell Factories·Alberto RodriguezFrancisco Bolivar
Dec 1, 2015·Journal of Applied Microbiology·Duolong ZhuMingqiang Qiao
Jan 20, 2015·Metabolic Engineering·Karthik Sekar, Keith E J Tyo
Sep 20, 2015·Metabolic Engineering·Shuang Ping LiuGui Yang Shi
Jan 3, 2014·Biotechnology and Bioengineering·Johan JarmanderGen Larsson
Oct 7, 2015·Frontiers in Bioengineering and Biotechnology·Juan Andrés MartínezAdelfo Escalante
Feb 12, 2014·Current Opinion in Microbiology·Ake Västermark, Milton H Saier
Apr 8, 2015·Journal of Proteomics·María Rodríguez-Moyá, Ramon Gonzalez
Aug 26, 2014·Frontiers in Bioengineering and Biotechnology·Andreas H Förster, Johannes Gescher
Sep 21, 2016·Applied Microbiology and Biotechnology·Vicente BernalManuel Cánovas
Jan 28, 2017·Nature Communications·Christopher P LongMaciek R Antoniewicz
Sep 16, 2016·Scientific Reports·Woongjae YooSangryeol Ryu
Jun 11, 2017·Biotechnology and Bioengineering·Todd C Chappell, Nikhil U Nair
Mar 21, 2018·Journal of Bacteriology·Jeremy T Ritzert, Wyndham W Lathem
Jun 22, 2018·Applied Microbiology and Biotechnology·Jingyu WangKechun Zhang
Jul 12, 2018·Molecular Microbiology·Sebastian BeblawyJohannes Gescher
Dec 20, 2017·Proceedings of the National Academy of Sciences of the United States of America·Christopher P LongMaciek R Antoniewicz
Sep 24, 2019·Critical Reviews in Biotechnology·Fernanda Karine do Carmo FélixCarlos Ricardo Soccol
May 6, 2019·PLoS Computational Biology·Anne GrimbsMarc-Thorsten Hütt
Jan 5, 2020·Applied Microbiology and Biotechnology·Alma AlvaFrancisco Bolívar
Aug 19, 2020·AMB Express·Yuki TatemichiMitsuyoshi Ueda
Jun 3, 2016·Clinical Science·Charles J DormanMatthew J Dorman
Nov 20, 2016·Scientific Reports·Laurence YangBernhard O Palsson
Sep 23, 2018·Microbial Cell Factories·Behrouz Mohammadi NargesiJung-Won Youn
Sep 23, 2018·BMC Systems Biology·Andreas KremlingHidde de Jong
Aug 5, 2017·World Journal of Microbiology & Biotechnology·Alba Romero-RodríguezSergio Sánchez
Aug 18, 2020·Applied and Environmental Microbiology·Peng LiuYu-Guo Zheng
Dec 29, 2020·Antimicrobial Agents and Chemotherapy·Sandra J AedoMark P Brynildsen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.