Current modulation and membrane targeting of the calcium channel alpha1C subunit are independent functions of the beta subunit

PMID: 10332087May 20, 1999Paper

Current modulation and membrane targeting of the calcium channel alpha1C subunit are independent functions of the beta subunit

The Journal of Physiology

U GersterB E Flucher

Abstract

1. The beta subunits of voltage-sensitive calcium channels facilitate the incorporation of channels into the plasma membrane and modulate calcium currents. In order to determine whether these two effects of the beta subunit are interdependent or independent of each other we studied plasma membrane incorporation of the channel subunits with green fluorescent protein and immunofluorescence labelling, and current modulation with whole-cell and single-channel patch-clamp recordings in transiently transfected human embryonic kidney tsA201 cells. 2. Coexpression of rabbit cardiac muscle alpha1C with rabbit skeletal muscle beta1a, rabbit heart/brain beta2a or rat brain beta3 subunits resulted in the colocalization of alpha1C with beta and in a marked translocation of the channel complexes into the plasma membrane. In parallel, the whole-cell current density and single-channel open probability were increased. Furthermore, the beta2a isoform specifically altered the voltage dependence of current activation and the inactivation kinetics. 3. A single amino acid substitution in the beta subunit interaction domain of alpha1C (alpha1CY467S) disrupted the colocalization and plasma membrane targeting of both subunits without affecting the beta...Continue Reading

References

The beta subunit controls the gating and dihydropyridine sensitivity of the skeletal muscle Ca2+ channel

Nov 1, 1992·Biophysical Journal·P LoryA Schwartz

Normalization of current kinetics by interaction between the alpha 1 and beta subunits of the skeletal muscle dihydropyridine-sensitive Ca2+ channel.

Aug 8, 1991·Nature·A E LacerdaA M Brown

Acceleration of activation and inactivation by the beta subunit of the skeletal muscle calcium channel

Jul 11, 1991·Nature·G VaradiA Schwartz

Subunit structure of dihydropyridine-sensitive calcium channels from skeletal muscle

Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M TakahashiW A Catterall

Primary structure and functional expression of the cardiac dihydropyridine-sensitive calcium channel.

Jul 20, 1989·Nature·A MikamiS Numa

Primary structure of the beta subunit of the DHP-sensitive calcium channel from skeletal muscle

Sep 8, 1989·Science·P RuthF Hofmann

Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches

Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth

Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis

Dec 1, 1983·Gene·J NorranderJ Messing

Calcium channel beta-subunit binds to a conserved motif in the I-II cytoplasmic linker of the alpha 1-subunit

Mar 3, 1994·Nature·M PragnellK P Campbell

Auxiliary subunits of voltage-gated ion channels

Jun 1, 1994·Neuron·L L IsomW A Catterall

Functional properties of cardiac L-type calcium channels transiently expressed in HEK293 cells. Roles of alpha 1 and beta subunits

Feb 1, 1995·The Journal of General Physiology·M T Pérez-GarcíaE Marbán

Properties of the alpha 1-beta anchoring site in voltage-dependent Ca2+ channels.

May 19, 1995·The Journal of Biological Chemistry·M De WaardK P Campbell

Improved green fluorescence

Feb 23, 1995·Nature·R HeimR Y Tsien

The naming of voltage-gated calcium channels

Sep 1, 1994·Neuron·L BirnbaumerT Tanabe

The amino terminus of a calcium channel beta subunit sets rates of channel inactivation independently of the subunit's effect on activation

Dec 1, 1994·Neuron·R OlceseL Birnbaumer

Ca2+ channel regulation by a conserved beta subunit domain

Aug 1, 1994·Neuron·M De WaardK P Campbell

Potentiation by the beta subunit of the ratio of the ionic current to the charge movement in the cardiac calcium channel

Oct 22, 1993·Science·A NeelyE Stefani

Triad formation: organization and function of the sarcoplasmic reticulum calcium release channel and triadin in normal and dysgenic muscle in vitro

Dec 1, 1993·The Journal of Cell Biology·B E FlucherJ A Powell

Single-channel analysis of a cloned human heart L-type Ca2+ channel alpha 1 subunit and the effects of a cardiac beta subunit

Nov 15, 1993·Biochemical and Biophysical Research Communications·M WakamoriA Yatani

Distinctive biophysical and pharmacological properties of class A (BI) calcium channel alpha 1 subunits

Aug 1, 1993·Neuron·W A SatherR W Tsien

The cloning and expression of a sodium channel beta 1-subunit cDNA from human brain

Jun 1, 1993·Human Molecular Genetics·A I McClatcheyJ F Gusella

Roles of a membrane-localized beta subunit in the formation and targeting of functional L-type Ca2+ channels.

Dec 15, 1995·The Journal of Biological Chemistry·A J ChienM M Hosey

Ca2+ current enhancement by alpha 2/delta and beta subunits in Xenopus oocytes: contribution of changes in channel gating and alpha 1 protein level

Nov 15, 1995·The Journal of Physiology·E ShistikN Dascal

Identification of critical amino acids involved in alpha1-beta interaction in voltage-dependent Ca2+ channels

Feb 19, 1996·FEBS Letters·M De WaardK P Campbell

Enhancement of ionic current and charge movement by coexpression of calcium channel beta 1A subunit with alpha 1C subunit in a human embryonic kidney cell line

Apr 1, 1996·The Journal of Physiology·T J KampE Marban

The beta subunit increases Ca2+ currents and gating charge movements of human cardiac L-type Ca2+ channels

Mar 1, 1996·Biophysical Journal·I R Josephson, G Varadi

Identification of palmitoylation sites within the L-type calcium channel beta2a subunit and effects on channel function.

Oct 25, 1996·The Journal of Biological Chemistry·A J ChienM M Hosey

Absence of the beta subunit (cchb1) of the skeletal muscle dihydropyridine receptor alters expression of the alpha 1 subunit and eliminates excitation-contraction coupling

Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·R G GreggP A Powers

Identification of a second region of the beta-subunit involved in regulation of calcium channel inactivation

Nov 1, 1996·The American Journal of Physiology·N QinE Stefani

A Xenopus oocyte beta subunit: evidence for a role in the assembly/expression of voltage-gated calcium channels that is separate from its role as a regulatory subunit

Mar 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·E TareilusL Birnbaumer

L-type calcium channels in insulin-secreting cells: biochemical characterization and phosphorylation in RINm5F cells

May 1, 1997·Molecular Endocrinology·H SafayhiJ Striessnig

Beta subunit heterogeneity in neuronal L-type Ca2+ channels.

May 23, 1997·The Journal of Biological Chemistry·M PichlerJ Striessnig

Importance of the different beta subunits in the membrane expression of the alpha1A and alpha2 calcium channel subunits: studies using a depolarization-sensitive alpha1A antibody

Apr 1, 1997·The European Journal of Neuroscience·N L BriceA C Dolphin

Subunit regulation of the human brain alpha 1E calcium channel

Nov 14, 1997·The Journal of Membrane Biology·L ParentD Talwar

A beta 4 isoform-specific interaction site in the carboxyl-terminal region of the voltage-dependent Ca2+ channel alpha 1A subunit.

Jan 27, 1998·The Journal of Biological Chemistry·D WalkerM De Waard

Tagging with green fluorescent protein reveals a distinct subcellular distribution of L-type and non-L-type Ca2+ channels expressed in dysgenic myotubes

Mar 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·M GrabnerK G Beam

Known calcium channel alpha1 subunits can form low threshold small conductance channels with similarities to native T-type channels

Mar 10, 1998·Neuron·A Meir, A C Dolphin

Differential effects of Ca2+ channel beta1a and beta2a subunits on complex formation with alpha1S and on current expression in tsA201 cells.

May 16, 1998·The Journal of Biological Chemistry·B NeuhuberB E Flucher

Unique regulatory properties of the type 2a Ca2+ channel beta subunit caused by palmitoylation

May 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·N QinL Birnbaumer

Association of calcium channel alpha1S and beta1a subunits is required for the targeting of beta1a but not of alpha1S into skeletal muscle triads

Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·B NeuhuberB E Flucher

Promotion and inhibition of L-type Ca2+ channel facilitation by distinct domains of the subunit.

Jul 11, 1998·The Journal of Biological Chemistry·T CensP Charnet

Multiple modulation pathways of calcium channel activity by a beta subunit. Direct evidence of beta subunit participation in membrane trafficking of the alpha1C subunit.

Jul 21, 1998·The Journal of Biological Chemistry·H YamaguchiG Varadi

❮ Previous

Next ❯

Citations

The voltage-gated calcium-channel beta subunit: more than just an accessory.

May 21, 2009·European Biophysics Journal : EBJ·Yamuna KarunasekaraMarco G Casarotto

Ca2+ currents in cardiac myocytes: Old story, new insights

Mar 1, 2006·Progress in Biophysics and Molecular Biology·Fabien BretteLaurent Sallé

Molecular characterization of a two-domain form of the neuronal voltage-gated P/Q-type calcium channel alpha(1)2.1 subunit

Dec 17, 2002·FEBS Letters·Jyothi ArikkathKevin P Campbell

Reciprocal interactions regulate targeting of calcium channel beta subunits and membrane expression of alpha1 subunits in cultured hippocampal neurons.

Dec 10, 2009·The Journal of Biological Chemistry·Gerald J ObermairBernhard E Flucher

Molecular determinants of the CaVbeta-induced plasma membrane targeting of the CaV1.2 channel.

May 19, 2010·The Journal of Biological Chemistry·Benoîte BourdinLucie Parent

Structural determinants of L-type channel activation in segment IIS6 revealed by a retinal disorder.

Sep 15, 2005·The Journal of Biological Chemistry·Annette HohausSteffen Hering

A novel Ca(V)1.2 N terminus expressed in smooth muscle cells of resistance size arteries modifies channel regulation by auxiliary subunits

Aug 19, 2007·The Journal of Biological Chemistry·Xiaoyang ChengJonathan H Jaggar

New Determinant for the CaVbeta2 subunit modulation of the CaV1.2 calcium channel.

Apr 16, 2008·The Journal of Biological Chemistry·Qi Zong LaoNikolai M Soldatov

Overexpressed Ca(v)beta3 inhibits N-type (Cav2.2) calcium channel currents through a hyperpolarizing shift of ultra-slow and closed-state inactivation

Mar 17, 2004·The Journal of General Physiology·Takahiro YasudaDavid J Adams

A CaVbeta SH3/guanylate kinase domain interaction regulates multiple properties of voltage-gated Ca2+ channels

Sep 28, 2005·The Journal of General Physiology·Shoji X TakahashiHenry M Colecraft

Regulation of maximal open probability is a separable function of Ca(v)beta subunit in L-type Ca2+ channel, dependent on NH2 terminus of alpha1C (Ca(v)1.2alpha)

Jun 28, 2006·The Journal of General Physiology·Nataly Kanevsky, Nathan Dascal

Molecular and electrophysiological differences in the L-type Ca2+ channel of the atrium and ventricle of rat hearts

Apr 26, 2006·Circulation Journal : Official Journal of the Japanese Circulation Society·Seiji HatanoLong-Tai Fu

Cooperation of two-domain Ca(2+) channel fragments in triad targeting and restoration of excitation- contraction coupling in skeletal muscle

Jul 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Bernhard E FlucherManfred Grabner

Modulation of the smooth-muscle L-type Ca2+ channel alpha1 subunit (alpha1C-b) by the beta2a subunit: a peptide which inhibits binding of beta to the I-II linker of alpha1 induces functional uncoupling

Jun 7, 2000·The Biochemical Journal·A HohausK Groschner

Transfer of beta subunit regulation from high to low voltage-gated Ca2+ channels

Jul 1, 2005·FEBS Letters·Juan Manuel AriasEdward Perez-Reyes

The alpha1B Ca2+ channel amino terminus contributes determinants for beta subunit-mediated voltage-dependent inactivation properties

Jun 2, 2000·The Journal of Physiology·G J StephensA C Dolphin

Calcium channels: when is a subunit not a subunit?

Nov 29, 2002·The Journal of Physiology·Stephen W Jones

The beta1a subunit regulates the functional properties of adult frog and mouse L-type Ca2+ channels of skeletal muscle

Nov 29, 2002·The Journal of Physiology·Rubén GarcíaJorge A Sánchez

The alpha 1-subunit of smooth muscle Ca(2+) channel preserves multiple open states induced by depolarization

Jul 6, 2000·The Journal of Physiology·S NakayamaM Kuzuya

TRP channel-associated factors are a novel protein family that regulates TRPM8 trafficking and activity

Jan 7, 2015·The Journal of Cell Biology·Dimitra GkikaNatalia Prevarskaya

Use of calcium channel blockers for glaucoma

Oct 12, 2010·Progress in Retinal and Eye Research·Makoto Araie, Chihiro Mayama

Vascular calcium channels and high blood pressure: pathophysiology and therapeutic implications

Jan 24, 2006·Vascular Pharmacology·Swapnil SonkusareNancy J Rusch

Calcium homeostasis in human placenta: role of calcium-handling proteins

Jul 25, 2006·International Review of Cytology·Julie Lafond, Lucie Simoneau

Functional expression of the L-type calcium channel in mice skeletal muscle during prenatal myogenesis

Feb 29, 2000·Biophysical Journal·C StrubeC Ojeda

The Kelch-like protein 1 modulates P/Q-type calcium current density

Feb 10, 2007·Neuroscience·K A AromolaranE S Piedras-Rentería

Single-molecule imaging of l-type Ca(2+) channels in live cells

Oct 19, 2001·Biophysical Journal·G S HarmsT Schmidt

Molecular heterogeneity of calcium channel beta-subunits in canine and human heart: evidence for differential subcellular localization

Feb 6, 2004·Physiological Genomics·Jason D FoellTimothy J Kamp

Differential targeting of the L-type Ca2+ channel alpha 1C (CaV1.2) to synaptic and extrasynaptic compartments in hippocampal neurons

Apr 20, 2004·The European Journal of Neuroscience·Gerald J ObermairBernhard E Flucher

A mutation in the beta interaction domain of the Ca(2+) channel alpha(1C) subunit reduces the affinity of the (+)-[(3)H]isradipine binding site

Jul 24, 2002·FEBS Letters·Monika HitzlBernhard E Flucher

Auxiliary subunit regulation of high-voltage activated calcium channels expressed in mammalian cells

Jul 13, 2004·The European Journal of Neuroscience·Takahiro YasudaGerald W Zamponi

Unique modulation of L-type Ca2+ channels by short auxiliary beta1d subunit present in cardiac muscle

Dec 24, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Risa M CohenTimothy J Kamp

Functional roles of the gamma subunit of the skeletal muscle DHP-receptor

Aug 10, 2006·Journal of Muscle Research and Cell Motility·Werner MelzerDaniel Ursu

Dominant-negative effects of episodic ataxia type 2 mutations involve disruption of membrane trafficking of human P/Q-type Ca2+ channels

Jul 27, 2007·Journal of Cellular Physiology·Chung-Jiuan JengChih-Yung Tang

A single CaVbeta can reconstitute both trafficking and macroscopic conductance of voltage-dependent calcium channels

Jul 16, 2005·The Journal of Physiology·Stanislava DaltonHenry M Colecraft

The role of auxiliary dihydropyridine receptor subunits in muscle

Aug 10, 2005·Journal of Muscle Research and Cell Motility·Bernhard E FlucherManfred Grabner

The importance of occupancy rather than affinity of CaV(beta) subunits for the calcium channel I-II linker in relation to calcium channel function.

Apr 22, 2006·The Journal of Physiology·Adrian J ButcherAnnette C Dolphin

RGK GTPase-dependent CaV2.1 Ca2+ channel inhibition is independent of CaVbeta-subunit-induced current potentiation

Apr 1, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J-P LeyrisP Charnet

Muscle-specific overexpression of IGF-I improves E-C coupling in skeletal muscle fibers from dystrophic mdx mice

Nov 9, 2007·American Journal of Physiology. Cell Physiology·Jonathan D SchertzerGordon S Lynch

The ß subunit of voltage-gated Ca2+ channels

Oct 21, 2010·Physiological Reviews·Zafir Buraei, Jian Yang

The carboxyl-terminal region of ahnak provides a link between cardiac L-type Ca2+ channels and the actin-based cytoskeleton

Aug 3, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Annette HohausHannelore Haase

Synaptic localization and presynaptic function of calcium channel beta 4-subunits in cultured hippocampal neurons

Aug 10, 2000·The Journal of Biological Chemistry·S WittemannS Herlitze

Voltage-gated mobility of the Ca2+ channel cytoplasmic tails and its regulatory role

Dec 11, 2002·The Journal of Biological Chemistry·Evgeny KobrinskyNikolai M Soldatov

Expression of voltage-gated Ca2+ channel subtypes in cultured astrocytes

Jan 30, 2003·Glia·Isabelle LatourBrian A Macvicar

A homozygous missense variant in CACNB4 encoding the auxiliary calcium channel beta4 subunit causes a severe neurodevelopmental disorder and impairs channel and non-channel functions

Mar 17, 2020·PLoS Genetics·Pierre Coste de BagneauxKerstin Kutsche

Competitive and synergistic interactions of G protein beta(2) and Ca(2+) channel beta(1b) subunits with Ca(v)2.1 channels, revealed by mammalian two-hybrid and fluorescence resonance energy transfer measurements

Sep 26, 2003·The Journal of Biological Chemistry·Alexander HümmerStefan Herlitze

Signaling from beta-adrenoceptor to L-type calcium channel: identification of a novel cardiac protein kinase A target possessing similarities to AHNAK

Dec 14, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·H HaaseI Morano

Regulation of cardiac and smooth muscle Ca(2+) channels (Ca(V)1.2a,b) by protein kinases

Nov 8, 2001·American Journal of Physiology. Cell Physiology·K D KeefJ Zhong

The C-terminal residues in the alpha-interacting domain (AID) helix anchor CaV beta subunit interaction and modulation of CaV2.3 channels

Oct 28, 2004·The Journal of Biological Chemistry·Laurent BerrouLucie Parent

Increased CaVbeta1A expression with aging contributes to skeletal muscle weakness.

Aug 12, 2009·Aging Cell·Jackson R TaylorOsvaldo Delbono

Properties of voltage-gated Ca(2+) channels in rabbit ventricular myocytes expressing Ca(2+) channel alpha(1E) cDNA

Dec 21, 2000·American Journal of Physiology. Cell Physiology·M TateyamaR Ochi

❮ Previous

Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.