Current Progression: Application of High-Throughput Sequencing Technique in Space Microbiology.

BioMed Research International
Yanwu ChenQiong Xie

Abstract

During a spaceflight, astronauts need to live in a spacecraft on orbit for a long time, and the relationship between humans and microorganisms in the closed environment of space is not the same as on the ground. The dynamic study of microorganisms in confined space shows that with the extension of the isolation time, harmful bacteria gradually accumulate. Monitoring and controlling microbial pollution in a confined environment system are very important for crew health and the sustainable operation of a space life support system. Culture-based assays have been used traditionally to assess the microbial loads in a spacecraft, and uncultured-based techniques are already under way according to the NASA global exploration roadmap. High-throughput sequencing technology has been used generally to study the communities of the environment and human on the ground and shows its broad prospects applied onboard. We here review the recent application of high-throughput sequencing on space microbiology and analyze its feasibility and potential as an on-orbit detection technology.

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Methods Mentioned

BETA
PCR
chip
chips
amplicon sequencing
ISS
Polymerase Chain Reaction

Software Mentioned

Solexa
PTS
MinION
Apollo
LOCAD

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