Current Status of Laboratory Diagnosis for COVID-19: A Narrative Review

Infection and Drug Resistance
Antonio RussoVanvitelli COVID-19 group

Abstract

The outbreak of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and the related disease (COVID-19) has spread rapidly to pandemic proportions, increasing the demands on healthcare systems for the containment and management of COVID-19. One of the critical issues to be addressed is the improvement in laboratory diagnosis and screening of large portions of the population to stop the virus spreading. Currently, the laboratory diagnosis of SARS-CoV-2 infection and the related disease is based on the research of viral RNA with rt-PCR methods in upper and lower respiratory airways. Serological tests to detect SARS-CoV-2 antibodies could help physicians and healthcare workers to support COVID-19 diagnosis and follow-up and perform population screening. Our review, using MEDLINE and EMBASE, summarizes the current knowledge of direct and serological tests performed to research RNA, antigens, or antibodies for SARS-CoV-2, evaluating the advantages and drawbacks for specific tests.

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Citations

Jan 28, 2021·Microorganisms·Aziz EftekhariMagali Cucchiarini
Dec 18, 2020·Frontiers in Plant Science·Arshak PoghossianMichael J Schöning
Dec 29, 2020·Infection and Drug Resistance·Antonio RussoUNKNOWN Vanvitelli-COVID-19 Group
Feb 19, 2021·Journal of Virological Methods·Joshua BlandShrihari S Kadkol
Feb 20, 2021·International Journal of General Medicine·Nahal EshghifarSafedin Beqaj
Mar 11, 2021·Journal of Multidisciplinary Healthcare·Shambel ArayaZemenu Tamir
Mar 13, 2021·Infection and Drug Resistance·Marco Giuseppe MigliaccioUNKNOWN Vanvitelli COVID-19 Group
Jul 18, 2020·International Journal of Environmental Research and Public Health·Margherita Macera Vanvitelli Covid-Group
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May 1, 2021·International Journal of Environmental Research and Public Health·Susanna EspositoUNKNOWN Working Group on COVID-19 in Pediatrics of the Emilia-Romagna Region (RE-CO-Ped)
May 1, 2021·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Bianca BruzzoneGiancarlo Icardi
Jun 25, 2021·Reviews in Medical Virology·Ranjita MisraNehru Sushmitha
Aug 6, 2021·Frontiers in Public Health·Dewi N AisyahZisis Kozlakidis
Aug 26, 2021·Infection and Drug Resistance·Ming-Jr JianHung-Sheng Shang
Sep 4, 2021·Expert Review of Anti-infective Therapy·Nihad AdnanMohd Raeed Jamiruddin
Sep 16, 2021·Genes & Genomics·Cherl-Joon LeeKyudong Han

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Methods Mentioned

BETA
transmission electron microscopy
glycosylation
PCR
enzyme-linked immune assay
ELISA
enzyme-linked immunosorbent assay
Assay

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