Current Strategies to Generate Human Mesenchymal Stem Cells In Vitro

Stem Cells International
Jennifer Steens, Diana Klein

Abstract

Mesenchymal stem cells (MSCs) are heterogeneous multipotent stem cells that are involved in the development of mesenchyme-derived evolving structures and organs during ontogeny. In the adult organism, reservoirs of MSCs can be found in almost all tissues where MSCs contribute to the maintenance of organ integrity. The use of these different MSCs for cell-based therapies has been extensively studied over the past years, which highlights the use of MSCs as a promising option for the treatment of various diseases including autoimmune and cardiovascular disorders. However, the proportion of MSCs contained in primary isolates of adult tissue biopsies is rather low and, thus, vigorous ex vivo expansion is needed especially for therapies that may require extensive and repetitive cell substitution. Therefore, more easily and accessible sources of MSCs are needed. This review summarizes the current knowledge of the different strategies to generate human MSCs in vitro as an alternative method for their applications in regenerative therapy.

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Citations

Apr 21, 2019·International Journal of Molecular Sciences·Ahmed Abdal DayemAnd Ssang-Goo Cho
Feb 23, 2019·Journal of Cellular Physiology·Mudasir Bashir GugjooGutulla Taru Sharma
Mar 18, 2020·Current Stem Cell Research & Therapy·Masoumeh AlishahiSeyed E Khoshnam
Dec 6, 2018·Journal of Cellular Physiology·Mudasir Bashir GugjooGutulla Taru Sharma
Apr 17, 2019·International Journal of Molecular Sciences·Felix UmrathMeltem Avci-Adali
Jun 7, 2019·World Journal of Stem Cells·Soo Kim, Tae Min Kim
Nov 13, 2019·Cellular and Molecular Life Sciences : CMLS·Jennifer SteensDiana Klein
Mar 3, 2019·Journal of Clinical Medicine·Stephen W Attwood, Michael J Edel
Dec 19, 2019·International Journal of Molecular Sciences·María Del Carmen Ortuño-CostelaM Esther Gallardo
Jan 23, 2021·Scientific Reports·C E AbanC Luzzani
Oct 28, 2020·International Journal of Molecular Sciences·Kasem TheerakittayakornRangsun Parnpai
Mar 7, 2021·International Journal of Molecular Sciences·Hantae JoBum-Ho Bin
Dec 31, 2020·International Journal of Stem Cells·Myung-Jin ChungKyu-Shik Jeong
Aug 18, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Manju ShresthaJee-Heon Jeong
Jan 16, 2022·Clinical Reviews in Allergy & Immunology·Xiufen ZhuangYanhong Wu

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Methods Mentioned

BETA
flow-activated cell sorting
FACS
flow cytometry
fluorescence-activated cell sorting
proteomic profiling

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