PMID: 16538029Mar 16, 2006Paper

Current topics in pharmacological research on bone metabolism: osteoclast differentiation regulated by glycosphingolipids

Journal of Pharmacological Sciences
Satoshi FukumotoYuzo Kato

Abstract

Glycosphingolipids are thought to play important roles in the development and function of several tissues, although the function of glycolipids in osteoclastogenesis has not been clearly demonstrated. In the present study, D-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol (D-PDMP), a glucosylceramide synthase inhibitor, completely inhibited osteoclastogenesis induced by macrophage-colony stimulating factor (M-CSF) and receptor activator of NF-kappaB ligand (RANKL). Following treatment with D-PDMP, nearly all glycosphingolipid expression was dramatically reduced on the surface of bone marrow cells, which suggests that glycosphingolipids are necessary for osteoclastogenesis. To determine which kinds of glycolipids are important for osteoclastogenesis, we added several types of purified glycolipids to D-PDMP treated bone marrow cells, as the precursor of osteoclasts is known to express glucosylceramide (GlcCer) and lactosylceramide (LacCer). Following treatment with RANKL, ganglioside GM3 and GM1 were increased in the treated bone marrow cells, whereas other types were not detected using thin layer chromatography analysis. In cells cultured with those glycolipids, exogenously added LacCer rescued osteoclastogenesis blocking...Continue Reading

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Citations

Dec 18, 2009·Human Molecular Genetics·Ying SunGregory A Grabowski
Jan 12, 2011·Wiener medizinische Wochenschrift·Peter Mikosch, Derralynn Hughes
Nov 6, 2007·Biological & Pharmaceutical Bulletin·Jae Youl Cho
Oct 8, 2013·Expert Opinion on Therapeutic Patents·Seong Hwan Kim, Seong-Hee Moon
Feb 13, 2009·Current Medical Research and Opinion·Gregory M PastoresAtul Mehta
Apr 15, 2016·Analytical and Bioanalytical Chemistry·Gang YangJian Zhang
Nov 27, 2014·Cellular and Molecular Life Sciences : CMLS·Zohreh Khavandgar, Monzur Murshed
Jan 10, 2021·Molecular Genetics and Metabolism·Gregory A GrabowskiPramod K Mistry

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